Endosomal SGLT1 and its possible role in the regulation of D-glucose uptake

Khoursandi, Saeed; Scharlau, Daniel; Kinne, Rolf K. H.; Kipp, Helmut

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Endosomal SGLT1 and its possible role in the regulation of D-glucose uptake

Khoursandi, S., Scharlau, D., Kinne, R. K. H., & Kipp, H. (2004). Endosomal SGLT1 and its possible role in the regulation of D-glucose uptake.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-0B89-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-0B8A-1

Genre: Conference Paper

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Creators:
Khoursandi, Saeed¹, Author
Scharlau, Daniel¹, Author
Kinne, Rolf K. H.², Author
Kipp, Helmut², Author

Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286
2Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294

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Abstract: To elucidate the role of endosomal SGLTl in the regulation of sodium-dependent D-glucose uptake into enterocytes, we investigated the relationship between subcellular distribution of SGLTl and sodium-dependent 14C-methylglucose uptake into Caco-2 cells under varying conditions. Incubation with mastoparan shifted a large amount of SGLTl from the apical membrane to intracellular sites and significantly reduced sodiumdependent 14C-methylglucose uptake. We also investigated the effect of extracellular D-glucose levels. Cells pre-incubated with D-glucose free medium exhibited a significantly higher sodiumdependent 14C-methylglucose uptake than cells pre-incubated with high D-glucose medium. No difference in the overall subcellular distribution of SGLTl was observed between the two conditions. Interestingly, the change in 14C-methylglucose uptake was attenuated when microtubules were depolymerized. These results suggest that pharmacologically, D-glucose uptake can be regulated by a shift of the steady state distribution of carriers from the plasma membrane to endosomes. The physiological substrate D-glucose alters substrate uptake by an additional mechanism that requires microtubule-dependent vesicle transport without a change in the steady state distribution. This phenomenon could be explained by the hypothesis that SGLTl undergoes an activation/deactivation cycle during its constitutive cycling from endosomes to the plasma membrane and back.

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Language(s): eng - English

Dates: Date issued: 2004-03

Publication Status: Issued

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Rev. Type: Internal

Identifiers: eDoc: 219423

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Title: Deutsche Physiologische Gesellschaft: 83rd Annual Meeting

Place of Event: Leipzig, Germany

Start-/End Date: 2004-03-14 - 2004-03-17

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Title: 83rd Annual Meeting DPG. Abstracts pp S1-S19

Source Genre: Issue

Creator(s):
Deten, A., Editor
Briest, W., Editor
Tag, H., Editor

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-

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Pages: Posterabstract Volume / Issue: - Sequence Number: P 23-5 Start / End Page: S 124 - S 124 Identifier: -

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Title: Pflügers Archiv European Journal of Physiology

Source Genre: Journal

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Pages: Posterabstract Volume / Issue: 447 (Supplement 1) Sequence Number: - Start / End Page: - Identifier: -