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  Endosomal SGLT1 and its possible role in the regulation of D-glucose uptake

Khoursandi, S., Scharlau, D., Kinne, R. K. H., & Kipp, H. (2004). Endosomal SGLT1 and its possible role in the regulation of D-glucose uptake.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-0B89-3 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-0B8A-1
Genre: Konferenzbeitrag

Externe Referenzen

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Urheber

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 Urheber:
Khoursandi, Saeed1, Autor
Scharlau, Daniel1, Autor
Kinne, Rolf K. H.2, Autor           
Kipp, Helmut2, Autor           
Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              
2Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294              

Inhalt

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Schlagwörter: -
 Zusammenfassung: To elucidate the role of endosomal SGLTl in the regulation of sodium-dependent D-glucose uptake into enterocytes, we investigated the relationship between subcellular distribution of SGLTl and sodium-dependent 14C-methylglucose uptake into Caco-2 cells under varying conditions. Incubation with mastoparan shifted a large amount of SGLTl from the apical membrane to intracellular sites and significantly reduced sodiumdependent 14C-methylglucose uptake. We also investigated the effect of extracellular D-glucose levels. Cells pre-incubated with D-glucose free medium exhibited a significantly higher sodiumdependent 14C-methylglucose uptake than cells pre-incubated with high D-glucose medium. No difference in the overall subcellular distribution of SGLTl was observed between the two conditions. Interestingly, the change in 14C-methylglucose uptake was attenuated when microtubules were depolymerized. These results suggest that pharmacologically, D-glucose uptake can be regulated by a shift of the steady state distribution of carriers from the plasma membrane to endosomes. The physiological substrate D-glucose alters substrate uptake by an additional mechanism that requires microtubule-dependent vesicle transport without a change in the steady state distribution. This phenomenon could be explained by the hypothesis that SGLTl undergoes an activation/deactivation cycle during its constitutive cycling from endosomes to the plasma membrane and back.

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Sprache(n): eng - English
 Datum: 2004-03
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Interne Begutachtung
 Identifikatoren: eDoc: 219423
 Art des Abschluß: -

Veranstaltung

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Titel: Deutsche Physiologische Gesellschaft: 83rd Annual Meeting
Veranstaltungsort: Leipzig, Germany
Start-/Enddatum: 2004-03-14 - 2004-03-17

Entscheidung

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Projektinformation

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Quelle 1

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Titel: 83rd Annual Meeting DPG. Abstracts pp S1-S19
Genre der Quelle: Heft
 Urheber:
Deten, A., Herausgeber
Briest, W., Herausgeber
Tag, H., Herausgeber
Affiliations:
-
Ort, Verlag, Ausgabe: -
Seiten: Posterabstract Band / Heft: - Artikelnummer: P 23-5 Start- / Endseite: S 124 - S 124 Identifikator: -

Quelle 2

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Titel: Pflügers Archiv European Journal of Physiology
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: Posterabstract Band / Heft: 447 (Supplement 1) Artikelnummer: - Start- / Endseite: - Identifikator: -