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  Exploring the effects of active site constraints on HIV-1 reverse transcriptase DNA polymerase fidelity

Cramer, J., Strerath, M., Marx, A., & Restle, T. (2002). Exploring the effects of active site constraints on HIV-1 reverse transcriptase DNA polymerase fidelity. Journal of Biological Chemistry, 277(46): 1, pp. 43593-43598. Retrieved from http://dx.doi.org/10.1074/jbc.M207854200.

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Genre: Journal Article
Alternative Title : J. Biol. Chem.

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 Creators:
Cramer, Janina1, Author
Strerath, Michael, Author
Marx, Andreas, Author
Restle, Tobias2, Author           
Affiliations:
1Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              
2Abt. III: Physikalische Biochemie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753289              

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 Abstract: To examine the concept of polymerase active site tightness as a criteria for DNA polymerase fidelity, we performed pre-steady- state single nucleotide incorporation kinetic analyses with sugar modified thymidine 5'- triphosphate (TTP) analogues and human immunodeficiency virus (HIV-1) reverse transcriptase (RT). The employed TTP analogues (TTP) are modified at the 4'- position of the sugar moiety with alkyl groups, gradually expanding their steric demand. Introduction of a methyl group reduces the maximum rate of nucleotide incorporation by about 200-fold for RTWT and about 400-fold for RTM184V. Interestingly, the affinity of RT for the modified nucleotide is only marginally affected. Increasing the size to an ethyl group leads to further reduction of the rate of incorporation and first effects on binding affinities are observed. Finally, substitution for an isopropyl group results not only in a further reduction of I. incorporation rates but also in a dramatic loss of bin,ding affinity for the nucleotide analogue. By increasing the steric demand the effects on RTM184V in comparison with RTWT become progressively more pronounced. Mis- incorporation of either TTP or (TTP)-T-Me opposite a template G causes additional decline in incorporation rates accompanied by a drastic decrease in binding affinities. This results in relative incorporation efficiencies [(k(pol)/K- d)(incorrect)/(k(pol)/K-d)(TTPcorrect)] of of 4.1 X 10(-5) for TTP and 3.4 X 10(-6) for (TTP)-T-Me in case of RTWT and 1.4 X 10(-5) for TTP and 2.9 X 10(-8) for (TTP)-T-Me in case of RTM184V.

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Language(s): eng - English
 Dates: 2002-11-15
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 13610
URI: http://dx.doi.org/10.1074/jbc.M207854200
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Title: Journal of Biological Chemistry
  Alternative Title : J. Biol. Chem.
Source Genre: Journal
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Pages: - Volume / Issue: 277 (46) Sequence Number: 1 Start / End Page: 43593 - 43598 Identifier: -