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  In caco-2 cells, most of the "apical" SGLT1 resides in intracellular, microtubuli-associated vesicles

Kipp, H., Khoursandi, S., & Kinne, R. K. H. (2002). In caco-2 cells, most of the "apical" SGLT1 resides in intracellular, microtubuli-associated vesicles.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-0EBA-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-0EBB-8
Genre: Conference Paper

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 Creators:
Kipp, Helmut1, Author              
Khoursandi, Saeed2, Author
Kinne, Rolf K. H.1, Author              
Affiliations:
1Sonstige Wissenschaftliche Organisationseinheiten, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753294              
2Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753286              

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 Abstract: We investigated the distribution of the endogenous sodium/D-glucose cotransporter (SGLT1) in polarized Caco-2 cells, a model for enterocytes. A cellular organelle fraction was separated by free flow electrophoresis. As detected by an ELISA assay the major amount of SGLT1 resides in early endosomal fractions and only a minor amount in apical plasma membranes. The distribution-ratio between endosomes and apical membrane was approximately 2:1. Further immunochemical investigation of SGLT1 distribution by epifluorescence and confocal microscopy revealed that SGLT1 is located in small vesicles associated with microtubuli. Furthermore, the half-life of SGLT1 in Caco-2 cells was determined to be 2.5 d by metabolic labeling followed by immunoprecipitation. Since SGLT1 has a relatively long residence time, it is unlikely that intracellular SGLT1 populations are part of the synthesis/degradation pathway. We, therefore, propose that intracellular compartments containing SGLT1 are part of an endo-/exocytosis process which regulates SGLT1 abundance at the apical cell surface in response to altered physiological demands for D-glucose reabsorption. (This abstract is as it is printed in the journal).

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Language(s): eng - English
 Dates: 2002-03-22
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: eDoc: 17910
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Title: Experimental Biology 2002
Place of Event: New Orleans, Louisiana, USA
Start-/End Date: 2002-04-20 - 2002-04-24

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Title: Abstracts Part II
Source Genre: Issue
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Pages: - Volume / Issue: - Sequence Number: 623.1 Start / End Page: A802 - A802 Identifier: -

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Title: FASEB Journal
Source Genre: Journal
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Pages: - Volume / Issue: 16 (5 (Part II)) Sequence Number: - Start / End Page: - Identifier: -