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  Transcription-dependent induction of G1 phase during the zebra fish midblastula transition

Zamir, E., Kam, Z., & Yarden, A. (1997). Transcription-dependent induction of G1 phase during the zebra fish midblastula transition. Molecular Cell Biology, 17(2), 529-536. Retrieved from http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=9001205.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1081-8 Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1082-6
Genre: Zeitschriftenartikel
Alternativer Titel : Mol Cell Biol

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 Urheber:
Zamir, E.1, Autor              
Kam, Z., Autor
Yarden, A., Autor
Affiliations:
1Abt. II: Systemische Zellbiologie, Max Planck Institute of Molecular Physiology, Max Planck Society, ou_1753288              

Inhalt

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Schlagwörter: Animals; Blastocyst/*cytology; Cell Cycle/physiology; DNA/biosynthesis/pharmacology; Dactinomycin/pharmacology; G1 Phase/*physiology; Microinjections; Nucleic Acid Synthesis Inhibitors/pharmacology; RNA/biosynthesis; Transcription, Genetic/*physiology; Zebrafish/embryology; Zygote
 Zusammenfassung: The early development of the zebra fish (Danio rerio) embryo is characterized by a series of rapid and synchronous cell cycles with no detectable transcription. This period is followed by the midblastula transition (MBT), during which the cell cycle gradually lengthens, cell synchrony is lost, and zygotic transcription is initially detected. In this work, we examined the changes in the pattern of the cell cycle during MBT in zebra fish and whether these changes are dependent on the initiation of zygotic transcription. To characterize the pattern of the early zebra fish cell cycles, the embryonic DNA content was determined by flow cytometric analysis. We found that G1 phase is below detection levels during the first 10 cleavages and can be initially detected at the onset of MBT. Inhibition of zygotic transcription, by microinjection of actinomycin D, abolished the appearance of G1 phase at MBT. Premature activation of zygotic transcription, by microinjection of nonspecific DNA, induced G1 phase before the onset of MBT, while coinjection of actinomycin D and nonspecific DNA abolished this early appearance of G1 phase. We therefore suggest that during the early development of the zebra fish embryo, G1 phase appears at the onset of MBT and that the activation of transcription at MBT is essential and sufficient for the G1-phase induction.

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 Datum: 1997-02
 Publikationsstatus: Im Druck publiziert
 Seiten: -
 Ort, Verlag, Ausgabe: -
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Titel: Molecular Cell Biology
  Alternativer Titel : Mol Cell Biol
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 17 (2) Artikelnummer: - Start- / Endseite: 529 - 536 Identifikator: ISSN: 0270-7306 (Print)