English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Serine acts as metabolic signal for the transcriptional control of photorespiration-related genes in Arabidopsis thaliana

Timm, S., Florian, A., Wittmiss, M., Jahnke, A., Hagemann, M., Fernie, A., et al. (2013). Serine acts as metabolic signal for the transcriptional control of photorespiration-related genes in Arabidopsis thaliana. Plant Physiology, 162(1), 379-389. doi:10.​1104/​pp.​113.​215970.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1D81-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1D82-F
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Timm, S.1, Author
Florian, A.2, Author              
Wittmiss, M.1, Author
Jahnke, A.1, Author
Hagemann, M.1, Author
Fernie, A.2, Author              
Bauwe, H.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: -
 Abstract: Photosynthetic carbon assimilation including photorespiration is dynamically regulated during the day/night cycle. This includes transcriptional regulation, such as the light induction of corresponding genes, but little is known about the contribution of photorespiratory metabolites to the regulation of gene expression. Here, we examined diurnal changes in the levels of photorespiratory metabolites, of enzymes of the photorespiratory carbon cycle, and of corresponding transcripts in wild-type plants of Arabidopsis (Arabidopsis thaliana) and in a mutant with altered photorespiratory flux due to the absence of the peroxisomal enzyme Hydroxypyruvate Reductase1 (HPR1). Metabolomics of the wild type showed that the relative amounts of most metabolites involved in photorespiration increased after the onset of light, exhibited maxima at the end of the day, and decreased during the night. In accordance with those findings, both the amounts of messenger RNAs encoding photorespiratory enzymes and the respective protein contents showed a comparable accumulation pattern. Deletion of HPR1 did not significantly alter most of the metabolite patterns relative to wild-type plants; only serine accumulated to a constitutively elevated amount in this mutant. In contrast, the hpr1 mutation resulted in considerable deregulation of the transcription of photorespiration-related genes. This transcriptional deregulation could also be induced by the external application of l-serine but not glycine to the Arabidopsis wild type, suggesting that serine acts as a metabolic signal for the transcriptional regulation of photorespiration, particularly in the glycine-to-serine interconversion reactions.

Details

show
hide
Language(s): eng - English
 Dates: 2013-03-092013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 23471132
DOI: 10.​1104/​pp.​113.​215970
ISSN: 0032-0889 (Print) 1532-2548 (Online)
URI: http://www.ncbi.nlm.nih.gov/pubmed/23471132
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 162 (1) Sequence Number: - Start / End Page: 379 - 389 Identifier: -