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  Chloroplast-localized 6-phosphogluconate dehydrogenase is critical for maize endosperm starch accumulation

Spielbauer, G., Li, L., Romisch-Margl, L., Do, P. T., Fouquet, R., Fernie, A. R., et al. (2013). Chloroplast-localized 6-phosphogluconate dehydrogenase is critical for maize endosperm starch accumulation. Journal of Experimental Botany, 64(8), 2231-2242. doi:10.1093/jxb/ert082.

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Spielbauer, G.1, Author
Li, L.1, Author
Romisch-Margl, L.1, Author
Do, P. T.2, Author           
Fouquet, R.1, Author
Fernie, A. R.2, Author           
Eisenreich, W.1, Author
Gierl, A.1, Author
Settles, A. M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Plants have duplicate versions of the oxidative pentose phosphate pathway (oxPPP) enzymes with a subset localized to the chloroplast. The chloroplast oxPPP provides NADPH and pentose sugars for multiple metabolic pathways. This study identified two loss-of-function alleles of the Zea mays (maize) chloroplast-localized oxPPP enzyme 6-phosphogluconate dehydrogenase (6PGDH). These mutations caused a rough endosperm seed phenotype with reduced embryo oil and endosperm starch. Genetic translocation experiments showed that pgd3 has separate, essential roles in both endosperm and embryo development. Endosperm metabolite profiling experiments indicated that pgd3 shifts redox-related metabolites and increases reducing sugars similar to starch-biosynthetis mutants. Heavy isotope-labelling experiments indicates that carbon flux into starch is altered in pgd3 mutants. Labelling experiments with a loss of cytosolic 6PGDH did not affect flux into starch. These results support the known role for plastid-localized oxPPP in oil synthesis and argue that amyloplast-localized oxPPP reactions are integral to endosperm starch accumulation in maize kernels.

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Language(s): eng - English
 Dates: 2013-03-272013
 Publication Status: Published in print
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 64 (8) Sequence Number: - Start / End Page: 2231 - 2242 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883