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  The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco

Gupta, K. J., Brotman, Y., Segu, S., Zeier, T., Zeier, J., Persijn, S. T., et al. (2013). The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco. Journal of Experimental Botany, 64(2), 553-868. doi:10.1093/jxb/ers348.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1DEF-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-C1F3-9
Genre: Journal Article

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Gupta, K. J.1, Author              
Brotman, Y.2, Author              
Segu, S.2, Author              
Zeier, T.1, Author
Zeier, J.1, Author
Persijn, S. T.1, Author
Cristescu, S. M.1, Author
Harren, F. J.1, Author
Bauwe, H.1, Author
Fernie, A. R.3, Author              
Kaiser, W. M.1, Author
Mur, L. A.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
3Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Different forms of nitrogen (N) fertilizer affect disease development; however, this study investigated the effects of N forms on the hypersensitivity response (HR)-a pathogen-elicited cell death linked to resistance. HR-eliciting Pseudomonas syringae pv. phaseolicola was infiltrated into leaves of tobacco fed with either or . The speed of cell death was faster in -fed compared with -fed plants, which correlated, respectively, with increased and decreased resistance. Nitric oxide (NO) can be generated by nitrate reductase (NR) to influence the formation of the HR. NO generation was reduced in -fed plants where N assimilation bypassed the NR step. This was similar to that elicited by the disease-forming P. syringae pv. tabaci strain, further suggesting that resistance was compromised with feeding. PR1a is a biomarker for the defence signal salicylic acid (SA), and expression was reduced in -fed compared with fed plants at 24h after inoculation. This pattern correlated with actual SA measurements. Conversely, total amino acid, cytosolic and apoplastic glucose/fructose and sucrose were elevated in - treated plants. Gas chromatography/mass spectroscopy was used to characterize metabolic events following different N treatments. Following nutrition, polyamine biosynthesis was predominant, whilst after nutrition, flux appeared to be shifted towards the production of 4-aminobutyric acid. The mechanisms whereby feeding enhances SA, NO, and polyamine-mediated HR-linked defence whilst these are compromised with , which also increases the availability of nutrients to pathogens, are discussed.

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Language(s): eng - English
 Dates: 2012-12-122013
 Publication Status: Published in print
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 64 (2) Sequence Number: - Start / End Page: 553 - 868 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883