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  Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato

de Godoy, F., Bermudez, L., Lira, B. S., de Souza, A. P., Elbl, P., Demarco, D., et al. (2013). Galacturonosyltransferase 4 silencing alters pectin composition and carbon partitioning in tomato. Journal of Experimental Botany, 64(8), 2449-2466. doi:10.1093/jxb/ert106.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1E17-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8071-1
Genre: Journal Article

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 Creators:
de Godoy, F.1, Author
Bermudez, L.1, Author
Lira, B. S.1, Author
de Souza, A. P.1, Author
Elbl, P.1, Author
Demarco, D.1, Author
Alseekh, S.2, Author              
Insani, M.1, Author
Buckeridge, M.1, Author
Almeida, J.1, Author
Grigioni, G.1, Author
Fernie, A. R.2, Author              
Carrari, F.1, Author              
Rossi, M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: Pectin is a main component of the plant cell wall and is the most complex family of polysaccharides in nature. Its composition is essential for the normal growth and morphology pattern, as demonstrated by pectin-defective mutant phenotypes. Besides this basic role in plant physiology, in tomato, pectin structure contributes to very important quality traits such as fruit firmness. Sixty-seven different enzymatic activities have been suggested to be required for pectin biosynthesis, but only a few genes have been identified and studied so far. This study characterized the tomato galacturonosyltransferase (GAUT) family and performed a detailed functional study of the GAUT4 gene. The tomato genome harbours all genes orthologous to those described previously in Arabidopsis thaliana, and a transcriptional profile revealed that the GAUT4 gene was expressed at higher levels in developing organs. GAUT4-silenced tomato plants exhibited an increment in vegetative biomass associated with palisade parenchyma enlargement. Silenced fruits showed an altered pectin composition and accumulated less starch along with a reduced amount of pectin, which coincided with an increase in firmness. Moreover, the harvest index was dramatically reduced as a consequence of the reduction in the fruit weight and number. Altogether, these results suggest that, beyond its role in pectin biosynthesis, GAUT4 interferes with carbon metabolism, partitioning, and allocation. Hence, this cell-wall-related gene seems to be key in determining plant growth and fruit production in tomato.

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Language(s): eng - English
 Dates: 2013-04-202013
 Publication Status: Published in print
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 64 (8) Sequence Number: - Start / End Page: 2449 - 2466 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883