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  Decreasing the Mitochondrial Synthesis of Malate in Potato Tubers Does Not Affect Plastidial Starch Synthesis, Suggesting That the Physiological Regulation of ADPglucose Pyrophosphorylase Is Context Dependent

Szecowka, M., Osorio, S., Obata, T., Araujo, W. L., Rohrmann, J., Nunes-Nesi, A., et al. (2012). Decreasing the Mitochondrial Synthesis of Malate in Potato Tubers Does Not Affect Plastidial Starch Synthesis, Suggesting That the Physiological Regulation of ADPglucose Pyrophosphorylase Is Context Dependent. Plant Physiology, 160(4), 2227-2238. doi:10.1104/pp.112.204826.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1E93-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1E94-E
Genre: Journal Article

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Szecowka, M.1, Author              
Osorio, S.1, Author              
Obata, T.1, Author              
Araujo, W. L.1, Author              
Rohrmann, J.1, Author              
Nunes-Nesi, A.1, Author              
Fernie, A. R.1, Author              
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1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: adp-glucose pyrophosphorylase adenosine 5'-diphosphoglucose pyrophosphorylase tomato fruit-development plant-growth molecular characterization antisense inhibition transcript levels enzyme-activities amino-acids root-growth
 Abstract: Modulation of the malate content of tomato (Solanum lycopersicum) fruit by altering the expression of mitochondrially localized enzymes of the tricarboxylic acid cycle resulted in enhanced transitory starch accumulation and subsequent effects on postharvest fruit physiology. In this study, we assessed whether such a manipulation would similarly affect starch biosynthesis in an organ that displays a linear, as opposed to a transient, kinetic of starch accumulation. For this purpose, we used RNA interference to down-regulate the expression of fumarase in potato (Solanum tuberosum) under the control of the tuber-specific B33 promoter. Despite displaying similar reductions in both fumarase activity and malate content as observed in tomato fruit expressing the same construct, the resultant transformants were neither characterized by an increased flux to, or accumulation of, starch, nor by alteration in yield parameters. Since the effect in tomato was mechanistically linked to derepression of the reaction catalyzed by ADP-glucose pyrophosphorylase, we evaluated whether the lack of effect on starch biosynthesis was due to differences in enzymatic properties of the enzyme from potato and tomato or rather due to differential subcellular compartmentation of reductant in the different organs. The results are discussed in the context both of current models of metabolic compartmentation and engineering.

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Language(s): eng - English
 Dates: 2012-10-162012
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000311998200043
DOI: 10.1104/pp.112.204826
ISSN: 1532-2548 (Electronic) 0032-0889 (Linking)
URI: ://000311998200043 http://www.plantphysiol.org/content/160/4/2227.full.pdf
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 160 (4) Sequence Number: - Start / End Page: 2227 - 2238 Identifier: -