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  Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by ectopic expression of a bacterial serine acetyltransferase

Nguyen, H. C., Hoefgen, R., & Hesse, H. (2012). Improving the nutritive value of rice seeds: elevation of cysteine and methionine contents in rice plants by ectopic expression of a bacterial serine acetyltransferase. Journal of Experimental Botany, 63(16), 5991-6001. doi:10.1093/Jxb/Ers253.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F1A-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F1B-9
Genre: Journal Article

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 Creators:
Nguyen, H. C.1, Author              
Hoefgen, R.1, Author              
Hesse, H.1, Author              
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1Amino Acid and Sulfur Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753337              

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Free keywords: cysteine and methionine enhancement nutritional improvement rice serine acetyltransferase cystathionine gamma-synthase transgenic potato plants amino-acids antisense inhibition pseudomonas-putida molecular-analysis aspartate kinase enhanced levels o-acetylserine organic sulfur
 Abstract: With the aim of increasing the cysteine level in rice (Oryza sativa L.) and thus improving its nutritional quality, transgenic rice plants were generated expressing an Escherichia coli serine acetyltransferase isoform (EcSAT), the enzyme synthesizing O-acetylserine, the precursor of cysteine. The gene was fused to the transit peptide of the Arabidopsis Rubisco and driven by a ubiquitin promoter to target the enzyme to plastids. Twenty-two transgenic plants were examined for transgene protein expression, and five lines with a high expression level and enzymatic activity, respectively, were selected for further analysis. In these lines, the contents of cysteine and glutathione increased 2.4-fold and 2-fold, respectively. More important is the increase in free methionine and methionine incorporated into the water-soluble protein fraction in seeds. Free methionine increased in leaves up to 2.7-fold, in seeds up to 1.4-fold, and bound to seed proteins up to 4.8-fold, respectively, while the bound methionine level remained constant or even decreased in leaves. Notably, the transgenic lines exhibited higher isoleucine, leucine, and valine contents (each up to 2-fold depending on tissue, free, or bound), indicating a potential conversion of methionine via methionine -lyase to isoleucine. As the transgenic rice plants overexpressing EcSAT had significantly higher levels of both soluble and protein-bound methionine, isoleucine, cysteine, and glutathione in rice they may represent a model and target system for improving the nutritional quality of cereal crops.

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Language(s): eng - English
 Dates: 2012-10-112012
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000309919500021
DOI: 10.1093/Jxb/Ers253
ISSN: 1460-2431 (Electronic) 0022-0957 (Linking)
URI: ://000309919500021 http://jxb.oxfordjournals.org/content/63/16/5991.full.pdf
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Title: Journal of Experimental Botany
Source Genre: Journal
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Pages: - Volume / Issue: 63 (16) Sequence Number: - Start / End Page: 5991 - 6001 Identifier: -