English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Metabolic recovery of Arabidopsis thaliana roots following cessation of oxidative stress

Lehmann, M., Laxa, M., Sweetlove, L. J., Fernie, A. R., & Obata, T. (2012). Metabolic recovery of Arabidopsis thaliana roots following cessation of oxidative stress. Metabolomics, 8(1), 143-153. doi:10.1007/s11306-011-0296-1.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F39-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8374-1
Genre: Journal Article

Files

show Files
hide Files
:
Lehmann-2012-Metabolic recovery o.pdf (Any fulltext), 441KB
Name:
Lehmann-2012-Metabolic recovery o.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Lehmann, M.1, Author              
Laxa, M.2, Author
Sweetlove, L. J.2, Author
Fernie, A. R.1, Author              
Obata, T.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: To cope with the various environmental stresses resulting in reactive oxygen species (ROS) production plant metabolism is known to be altered specifically under different stresses. After overcoming the stress the metabolism should be reconfigured to recover basal operation however knowledge concerning how this is achieved is cursory. To investigate the metabolic recovery of roots following oxidative stress, changes in metabolite abundance and carbon flow were analysed. Arabidopsis roots were treated by menadione to elicit oxidative stress. Roots were fed with (13)C labelled glucose and the redistribution of isotope was determined in order to study carbon flow. The label redistribution through many pathways such as glycolysis, the tricarboxylic acid (TCA) cycle and amino acid metabolism were reduced under oxidative stress. After menadione removal many of the stress-related changes reverted back to basal levels. Decreases in amounts of hexose phosphates, malate, 2-oxoglutarate, glutamate and aspartate were fully recovered or even increased to above the control level. However, some metabolites such as pentose phosphates and citrate did not recover but maintained their levels or even increased further. The alteration in label redistribution largely correlated with that in metabolite abundance. Glycolytic carbon flow reverted to the control level only 18 h after menadione removal although the TCA cycle and some amino acids such as aspartate and glutamate took longer to recover. Taken together, plant root metabolism was demonstrated to be able to overcome menadione-induced oxidative stress with the differential time period required by independent pathways suggestive of the involvement of pathway specific regulatory processes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-011-0296-1) contains supplementary material, which is available to authorized users.

Details

show
hide
Language(s): eng - English
 Dates: 2011-03-112012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Metabolomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Springer
Pages: - Volume / Issue: 8 (1) Sequence Number: - Start / End Page: 143 - 153 Identifier: Other: 1573-3882
CoNE: https://pure.mpg.de/cone/journals/resource/1573-3882