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  Comparison of freezing tolerance, compatible solutes and polyamines in geographically diverse collections of Thellungiella sp. and Arabidopsis thaliana accessions

Lee, Y. P., Babakov, A., de Boer, B., Zuther, E., & Hincha, D. K. (2012). Comparison of freezing tolerance, compatible solutes and polyamines in geographically diverse collections of Thellungiella sp. and Arabidopsis thaliana accessions. BMC Plant Biology, 12, 131. doi:10.1186/1471-2229-12-131.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F3C-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F3D-E
Genre: Journal Article

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 Creators:
Lee, Y. P.1, Author              
Babakov, A.2, Author
de Boer, B.2, Author
Zuther, E.1, Author              
Hincha, D. K.1, Author              
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1Transcript Profiling, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753306              
2External Organizations, ou_persistent22              

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Free keywords: arabidopsis thaliana cold acclimation compatible solutes freezing tolerance natural variation polyamines thellungiella salsuginea cold-acclimation response natural variation gene-expression low-temperature abiotic stress plants halophila heterosis proline genome
 Abstract: Background: Thellungiella has been proposed as an extremophile alternative to Arabidopsis to investigate environmental stress tolerance. However, Arabidopsis accessions show large natural variation in their freezing tolerance and here the tolerance ranges of collections of accessions in the two species were compared. Results: Leaf freezing tolerance of 16 Thellungiella accessions was assessed with an electrolyte leakage assay before and after 14 days of cold acclimation at 4 degrees C. Soluble sugars (glucose, fructose, sucrose, raffinose) and free polyamines (putrescine, spermidine, spermine) were quantified by HPLC, proline photometrically. The ranges in nonacclimated freezing tolerance completely overlapped between Arabidopsis and Thellungiella. After cold acclimation, some Thellungiella accessions were more freezing tolerant than any Arabidopsis accessions. Acclimated freezing tolerance was correlated with sucrose levels in both species, but raffinose accumulation was lower in Thellungiella and only correlated with freezing tolerance in Arabidopsis. The reverse was true for leaf proline contents. Polyamine levels were generally similar between the species. Only spermine content was higher in nonacclimated Thellungiella plants, but decreased during acclimation and was negatively correlated with freezing tolerance. Conclusion: Thellungiella is not an extremophile with regard to freezing tolerance, but some accessions significantly expand the range present in Arabidopsis. The metabolite data indicate different metabolic adaptation strategies between the species.

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Language(s): eng - English
 Dates: 2012-08-072012
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000308695600001
DOI: 10.1186/1471-2229-12-131
ISSN: 1471-2229 (Electronic) 1471-2229 (Linking)
URI: ://000308695600001 http://www.biomedcentral.com/1471-2229/12/131 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464606/pdf/1471-2229-12-131.pdf
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Title: BMC Plant Biology
Source Genre: Journal
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Pages: - Volume / Issue: 12 Sequence Number: - Start / End Page: 131 Identifier: -