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  Multiple strategies to prevent oxidative stress in Arabidopsis plants lacking the malate valve enzyme NADP-malate dehydrogenase

Hebbelmann, I., Selinski, J., Wehmeyer, C., Goss, T., Voss, I., Mulo, P., et al. (2012). Multiple strategies to prevent oxidative stress in Arabidopsis plants lacking the malate valve enzyme NADP-malate dehydrogenase. Journal of Experimental Botany, 63(3), 1445-1459. doi:10.1093/jxb/err386.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1F94-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8378-A
Genre: Journal Article

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 Creators:
Hebbelmann, I.1, Author
Selinski, J.1, Author
Wehmeyer, C.1, Author
Goss, T.1, Author
Voss, I.1, Author
Mulo, P.1, Author
Kangasjarvi, S.1, Author
Aro, E.-M.1, Author
Oelze, M. L.1, Author
Dietz, K. J.1, Author
Nunes-Nesi, A.2, Author              
Do, P. T.2, Author              
Fernie, A. R.2, Author              
Talla, S. K.1, Author
Raghavendra, A. S.1, Author
Linke, V.1, Author
Scheibe, R.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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 Abstract: The nuclear-encoded chloroplast NADP-dependent malate dehydrogenase (NADP-MDH) is a key enzyme controlling the malate valve, to allow the indirect export of reducing equivalents. Arabidopsis thaliana (L.) Heynh. T-DNA insertion mutants of NADP-MDH were used to assess the role of the light-activated NADP-MDH in a typical C(3) plant. Surprisingly, even when exposed to high-light conditions in short days, nadp-mdh knockout mutants were phenotypically indistinguishable from the wild type. The photosynthetic performance and typical antioxidative systems, such as the Beck-Halliwell-Asada pathway, were barely affected in the mutants in response to high-light treatment. The reactive oxygen species levels remained low, indicating the apparent absence of oxidative stress, in the mutants. Further analysis revealed a novel combination of compensatory mechanisms in order to maintain redox homeostasis in the nadp-mdh plants under high-light conditions, particularly an increase in the NTRC/2-Cys peroxiredoxin (Prx) system in chloroplasts. There were indications of adjustments in extra-chloroplastic components of photorespiration and proline levels, which all could dissipate excess reducing equivalents, sustain photosynthesis, and prevent photoinhibition in nadp-mdh knockout plants. Such metabolic flexibility suggests that the malate valve acts in concert with other NADPH-consuming reactions to maintain a balanced redox state during photosynthesis under high-light stress in wild-type plants.

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Language(s): eng - English
 Dates: 2011-12-062012
 Publication Status: Published in print
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Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
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Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 63 (3) Sequence Number: - Start / End Page: 1445 - 1459 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883