English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A "Crossomics" Study Analysing Variability of Different Components in Peripheral Blood of Healthy Caucasoid Individuals

Gruden, K., Hren, M., Herman, A., Blejec, A., Albrecht, T., Selbig, J., et al. (2012). A "Crossomics" Study Analysing Variability of Different Components in Peripheral Blood of Healthy Caucasoid Individuals. PLoS One, 7(1), e28761. doi:10.1371/journal.pone.0028761.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1FB0-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1FB1-4
Genre: Journal Article

Files

show Files
hide Files
:
Gruden-2012-A _Crossomics_ Study.pdf (Any fulltext), 558KB
Name:
Gruden-2012-A _Crossomics_ Study.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Gruden, K.1, Author
Hren, M.1, Author
Herman, A.1, Author
Blejec, A.1, Author
Albrecht, T.1, Author
Selbig, J.2, Author              
Bauer, C.1, Author
Schuchardt, J.1, Author
Or-Guil, M.1, Author
Zupancic, K.1, Author
Svajger, U.1, Author
Stabuc, B.1, Author
Ihan, A.1, Author
Kopitar, A. N.1, Author
Ravnikar, M.1, Author
Knezevic, M.1, Author
Rozman, P.1, Author
Jeras, M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2BioinformaticsCRG, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753315              

Content

show
hide
Free keywords: messenger-rna expression gene-expression whole-blood reveals differences autoimmune-disease immune-system cells serum profiles cytokine
 Abstract: Background: Different immunotherapy approaches for the treatment of cancer and autoimmune diseases are being developed and tested in clinical studies worldwide. Their resulting complex experimental data should be properly evaluated, therefore reliable normal healthy control baseline values are indispensable. Methodology/Principal Findings: To assess intra- and inter-individual variability of various biomarkers, peripheral blood of 16 age and gender equilibrated healthy volunteers was sampled on 3 different days within a period of one month. Complex "crossomics'' analyses of plasma metabolite profiles, antibody concentrations and lymphocyte subset counts as well as whole genome expression profiling in CD4(+)T and NK cells were performed. Some of the observed age, gender and BMI dependences are in agreement with the existing knowledge, like negative correlation between sex hormone levels and age or BMI related increase in lipids and soluble sugars. Thus we can assume that the distribution of all 39.743 analysed markers is well representing the normal Caucasoid population. All lymphocyte subsets, 20% of metabolites and less than 10% of genes, were identified as highly variable in our dataset. Conclusions/Significance: Our study shows that the intra- individual variability was at least two-fold lower compared to the inter-individual one at all investigated levels, showing the importance of personalised medicine approach from yet another perspective.

Details

show
hide
Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000301357100004
DOI: 10.1371/journal.pone.0028761
ISSN: 1932-6203
URI: ://000301357100004 http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0028761&representation=PDF
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (1) Sequence Number: - Start / End Page: e28761 Identifier: -