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  Downregulation of the delta-Subunit Reduces Mitochondrial ATP Synthase Levels, Alters Respiration, and Restricts Growth and Gametophyte Development in Arabidopsis

Geisler, D. A., Päpke, C., Obata, T., Nunes-Nesi, A., Matthes, A., Schneitz, K., et al. (2012). Downregulation of the delta-Subunit Reduces Mitochondrial ATP Synthase Levels, Alters Respiration, and Restricts Growth and Gametophyte Development in Arabidopsis. The Plant Cell, 24(7), 2792-2811. doi:10.1105/tpc.112.099424.

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Geisler, D. A.1, Autor           
Päpke, C.1, Autor           
Obata, T.2, Autor           
Nunes-Nesi, A.2, Autor           
Matthes, A.3, Autor           
Schneitz, K.4, Autor
Maximova, E.5, Autor           
Araujo, W. L.2, Autor           
Fernie, A. R.2, Autor           
Persson, S.1, Autor           
Affiliations:
1Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
4External Organizations, ou_persistent22              
5Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753304              

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Schlagwörter: alternative nad(p)h dehydrogenases complex-i plant-mitochondria electron-transport pollen development oxidative stress gene-expression photosynthetic performance nicotiana-sylvestris antisense repression
 Zusammenfassung: The mitochondrial ATP synthase (F1Fo complex) is an evolutionary conserved multimeric protein complex that synthesizes the main bulk of cytosolic ATP, but the regulatory mechanisms of the subunits are only poorly understood in plants. In yeast, the delta-subunit links the membrane-embedded F-o part to the matrix-facing central stalk of F-1. We used genetic interference and an inhibitor to investigate the molecular function and physiological impact of the delta-subunit in Arabidopsis thaliana. Delta mutants displayed both male and female gametophyte defects. RNA interference of delta resulted in growth retardation, reduced ATP synthase amounts, and increased alternative oxidase capacity and led to specific long-term increases in Ala and Gly levels. By contrast, inhibition of the complex using oligomycin triggered broad metabolic changes, affecting glycolysis and the tricarboxylic acid cycle, and led to a successive induction of transcripts for alternative respiratory pathways and for redox and biotic stress-related transcription factors. We conclude that (1) the delta-subunit is essential for male gametophyte development in Arabidopsis, (2) a disturbance of the ATP synthase appears to lead to an early transition phase and a long-term metabolic steady state, and (3) the observed long-term adjustments in mitochondrial metabolism are linked to reduced growth and deficiencies in gametophyte development.

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Sprache(n): eng - English
 Datum: 2012-07-192012
 Publikationsstatus: Erschienen
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 Identifikatoren: ISI: ISI:000308352800010
DOI: 10.1105/tpc.112.099424
ISSN: 1532-298X (Electronic)1040-4651 (Linking)
URI: ://000308352800010http://www.plantcell.org/content/24/7/2792.full.pdf
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Titel: The Plant Cell
  Kurztitel : Plant C
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Rockville : American Society of Plant Physiologists
Seiten: - Band / Heft: 24 (7) Artikelnummer: - Start- / Endseite: 2792 - 2811 Identifikator: Anderer: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X