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  Downregulation of the delta-Subunit Reduces Mitochondrial ATP Synthase Levels, Alters Respiration, and Restricts Growth and Gametophyte Development in Arabidopsis

Geisler, D. A., Päpke, C., Obata, T., Nunes-Nesi, A., Matthes, A., Schneitz, K., et al. (2012). Downregulation of the delta-Subunit Reduces Mitochondrial ATP Synthase Levels, Alters Respiration, and Restricts Growth and Gametophyte Development in Arabidopsis. The Plant Cell, 24(7), 2792-2811. doi:10.1105/tpc.112.099424.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-1FC1-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-816F-1
Genre: Journal Article

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 Creators:
Geisler, D. A.1, Author              
Päpke, C.1, Author              
Obata, T.2, Author              
Nunes-Nesi, A.2, Author              
Matthes, A.3, Author              
Schneitz, K.4, Author
Maximova, E.5, Author              
Araujo, W. L.2, Author              
Fernie, A. R.2, Author              
Persson, S.1, Author              
Affiliations:
1Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
3Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
4External Organizations, ou_persistent22              
5Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753304              

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Free keywords: alternative nad(p)h dehydrogenases complex-i plant-mitochondria electron-transport pollen development oxidative stress gene-expression photosynthetic performance nicotiana-sylvestris antisense repression
 Abstract: The mitochondrial ATP synthase (F1Fo complex) is an evolutionary conserved multimeric protein complex that synthesizes the main bulk of cytosolic ATP, but the regulatory mechanisms of the subunits are only poorly understood in plants. In yeast, the delta-subunit links the membrane-embedded F-o part to the matrix-facing central stalk of F-1. We used genetic interference and an inhibitor to investigate the molecular function and physiological impact of the delta-subunit in Arabidopsis thaliana. Delta mutants displayed both male and female gametophyte defects. RNA interference of delta resulted in growth retardation, reduced ATP synthase amounts, and increased alternative oxidase capacity and led to specific long-term increases in Ala and Gly levels. By contrast, inhibition of the complex using oligomycin triggered broad metabolic changes, affecting glycolysis and the tricarboxylic acid cycle, and led to a successive induction of transcripts for alternative respiratory pathways and for redox and biotic stress-related transcription factors. We conclude that (1) the delta-subunit is essential for male gametophyte development in Arabidopsis, (2) a disturbance of the ATP synthase appears to lead to an early transition phase and a long-term metabolic steady state, and (3) the observed long-term adjustments in mitochondrial metabolism are linked to reduced growth and deficiencies in gametophyte development.

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Language(s): eng - English
 Dates: 2012-07-192012
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000308352800010
DOI: 10.1105/tpc.112.099424
ISSN: 1532-298X (Electronic)1040-4651 (Linking)
URI: ://000308352800010http://www.plantcell.org/content/24/7/2792.full.pdf
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 24 (7) Sequence Number: - Start / End Page: 2792 - 2811 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X