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  Functional associations between the metabolome and manganese tolerance in Vigna unguiculata

Fuehrs, H., Specht, A., Erban, A., Kopka, J., & Horst, W. J. (2012). Functional associations between the metabolome and manganese tolerance in Vigna unguiculata. Journal of Experimental Botany, 63(1), 329-340. doi:10.1093/Jxb/Err276.

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 Creators:
Fuehrs, H.1, Author
Specht, A.1, Author
Erban, A.2, Author              
Kopka, J.2, Author              
Horst, W. J.1, Author
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1External Organizations, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

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Free keywords: antioxidative capacity metabolomics mn chelation shikimate and phenylpropanoid metabolism gas-chromatography mass-spectrometry leaf apoplast phanerochaete-chrysosporium transcriptomic analysis silicon cowpea toxicity stress plants
 Abstract: Genotypic- and silicon (Si)-mediated differences in manganese (Mn) tolerance of cowpea (Vigna unguiculata) arise from a combination of symplastic and apoplastic traits. A detailed metabolomic inspection could help to identify functional associations between genotype- and Si-mediated Mn tolerance and metabolism. Two cowpea genotypes differing in Mn tolerance (TVu 91, Mn sensitive; TVu 1987, Mn tolerant) were subjected to differential Mn and Si treatments. Gas chromatography-mass spectrometry (GC-MS)-based metabolite profiling of leaf material was performed. Detailed evaluation of the response of metabolites was combined with gene expression and physiological analyses. After 2 d of 50 mu M Mn supply TVu 91 expressed toxicity symptoms first in the form of brown spots on the second oldest trifoliate leaves. Silicon treatment suppressed symptom development in TVu 91. Despite higher concentrations of Mn in leaves of TVu 1987 compared with TVu 91, the tolerant genotype did not show symptoms. From sample cluster formation as identified by independent component analysis (ICA) of metabolite profiles it is concluded that genotypic differences accounted for the highest impact on variation in metabolite pools, followed by Mn and Si treatments in one of two experiments. Analysis of individual metabolites corroborated a comparable minor role for Mn and Si treatments in the modulation of individual metabolites. Mapping individual metabolites differing significantly between genotypes onto biosynthetic pathways and gene expression studies on the corresponding pathways suggest that genotypic Mn tolerance is a consequence of differences (i) in the apoplastic binding capacity; (ii) in the capability to maintain a high antioxidative state; and (iii) in the activity of shikimate and phenylpropanoid metabolism.

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Language(s): eng - English
 Dates: 2011-09-202012
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000298383400026
DOI: 10.1093/Jxb/Err276
ISSN: 1460-2431 (Electronic) 0022-0957 (Linking)
URI: ://000298383400026 http://jxb.oxfordjournals.org/content/63/1/329.full.pdf
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Title: Journal of Experimental Botany
Source Genre: Journal
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Pages: - Volume / Issue: 63 (1) Sequence Number: - Start / End Page: 329 - 340 Identifier: -