English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Expression of ROS-responsive genes and transcription factors after metabolic formation of H(2)O(2) in chloroplasts

Balazadeh, S., Jaspert, N., Arif, M., Mueller-Roeber, B., & Maurino, V. G. (2012). Expression of ROS-responsive genes and transcription factors after metabolic formation of H(2)O(2) in chloroplasts. Frontiers in plant science, 3: 234. doi:10.3389/fpls.2012.00234.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-204D-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-732D-A
Genre: Journal Article

Files

show Files
hide Files
:
Balazadeh-2012-Expression of ROS-re.pdf (Any fulltext), 2MB
Name:
Balazadeh-2012-Expression of ROS-re.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Balazadeh, S.1, Author              
Jaspert, N.2, Author
Arif, M.1, Author              
Mueller-Roeber, B.3, Author              
Maurino, V. G.1, Author              
Affiliations:
1Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              
2External Organizations, ou_persistent22              
3Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              

Content

show
hide
Free keywords: -
 Abstract: Glycolate oxidase (GO) catalyses the oxidation of glycolate to glyoxylate, thereby consuming O(2) and producing H(2)O(2). In this work, Arabidopsis thaliana plants expressing GO in the chloroplasts (GO plants) were used to assess the expressional behavior of reactive oxygen species (ROS)-responsive genes and transcription factors (TFs) after metabolic induction of H(2)O(2) formation in chloroplasts. In this organelle, GO uses the glycolate derived from the oxygenase activity of RubisCO. Here, to identify genes responding to an abrupt production of H(2)O(2) in chloroplasts we used quantitative real-time PCR (qRT-PCR) to test the expression of 187 ROS-responsive genes and 1880 TFs after transferring GO and wild-type (WT) plants grown at high CO(2) levels to ambient CO(2) concentration. Our data revealed coordinated expression changes of genes of specific functional networks 0.5 h after metabolic induction of H(2)O(2) production in GO plants, including the induction of indole glucosinolate and camalexin biosynthesis genes. Comparative analysis using available microarray data suggests that signals for the induction of these genes through H(2)O(2) may originate in the chloroplast. The TF profiling indicated an up-regulation in GO plants of a group of genes involved in the regulation of proanthocyanidin and anthocyanin biosynthesis. Moreover, the upregulation of expression of TF and TF-interacting proteins affecting development (e.g., cell division, stem branching, flowering time, flower development) would impact growth and reproductive capacity, resulting in altered development under conditions that promote the formation of H(2)O(2).

Details

show
hide
Language(s): eng - English
 Dates: 2012-11-062012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Frontiers in plant science
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 Sequence Number: 234 Start / End Page: - Identifier: -