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  Optical Oxygen Micro- and Nanosensors for Plant Applications

Ast, C., Schmalzlin, E., Lohmannsroben, H. G., & van Dongen, J. T. (2012). Optical Oxygen Micro- and Nanosensors for Plant Applications. Sensors, 12(6), 7015-7032. doi:10.3390/S120607015.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2056-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2057-B
Genre: Journal Article

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Ast-2012-Optical Oxygen Micro.pdf (Any fulltext), 345KB
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 Creators:
Ast, C.1, Author              
Schmalzlin, E.2, Author
Lohmannsroben, H. G.2, Author
van Dongen, J. T.1, Author              
Affiliations:
1Energy Metabolism, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753325              
2External Organizations, ou_persistent22              

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Free keywords: oxygen sensor biosensors microsensors nanosensors endogenous sensor proteins dual-frequency phase-modulation phosphorescence quenching plant science cell-penetrating peptides green fluorescent protein intracellular delivery phosphorescent probes internal oxygen sensor gradients binding state permeability
 Abstract: Pioneered by Clark's microelectrode more than half a century ago, there has been substantial interest in developing new, miniaturized optical methods to detect molecular oxygen inside cells. While extensively used for animal tissue measurements, applications of intracellular optical oxygen biosensors are still scarce in plant science. A critical aspect is the strong autofluorescence of the green plant tissue that interferes with optical signals of commonly used oxygen probes. A recently developed dual-frequency phase modulation technique can overcome this limitation, offering new perspectives for plant research. This review gives an overview on the latest optical sensing techniques and methods based on phosphorescence quenching in diverse tissues and discusses the potential pitfalls for applications in plants. The most promising oxygen sensitive probes are reviewed plus different oxygen sensing structures ranging from micro-optodes to soluble nanoparticles. Moreover, the applicability of using heterologously expressed oxygen binding proteins and fluorescent proteins to determine changes in the cellular oxygen concentration are discussed as potential non-invasive cellular oxygen reporters.

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Language(s): eng - English
 Dates: 2012
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000305801400014
DOI: 10.3390/S120607015
ISSN: 1424-8220
URI: ://000305801400014 http://www.mdpi.com/1424-8220/12/6/7015/pdf
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Title: Sensors
Source Genre: Journal
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Pages: - Volume / Issue: 12 (6) Sequence Number: - Start / End Page: 7015 - 7032 Identifier: -