English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Antisense Inhibition of the 2-Oxoglutarate Dehydrogenase Complex in Tomato Demonstrates Its Importance for Plant Respiration and during Leaf Senescence and Fruit Maturation

Araujo, W. L., Tohge, T., Osorio, S., Lohse, M., Balbo, I., Krahnert, I., et al. (2012). Antisense Inhibition of the 2-Oxoglutarate Dehydrogenase Complex in Tomato Demonstrates Its Importance for Plant Respiration and during Leaf Senescence and Fruit Maturation. The Plant Cell, 24(6), 2328-2351. doi:10.1105/tpc.112.099002.

Item is

Files

show Files
hide Files
:
Araujo-2012-Antisense Inhibition.pdf (Any fulltext), 2MB
Name:
Araujo-2012-Antisense Inhibition.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Araujo, W. L.1, Author              
Tohge, T.1, Author              
Osorio, S.1, Author              
Lohse, M.2, Author              
Balbo, I.1, Author              
Krahnert, I.1, Author              
Sienkiewicz-Porzucek, A.1, Author              
Usadel, B.2, Author              
Nunes-Nesi, A.1, Author              
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              

Content

show
hide
Free keywords: tricarboxylic-acid cycle mitochondrial electron-transport dark-induced senescence iron-sulfur subunit nicotiana-sylvestris abscisic-acid nitrogen assimilation illuminated leaves gibberellic-acid isocitrate dehydrogenases
 Abstract: Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the gene encoding the E1 subunit of the 2-oxoglutarate dehydrogenase complex in the antisense orientation and exhibiting substantial reductions in the activity of this enzyme exhibit a considerably reduced rate of respiration. They were, however, characterized by largely unaltered photosynthetic rates and fruit yields but restricted leaf, stem, and root growth. These lines displayed markedly altered metabolic profiles, including changes in tricarboxylic acid cycle intermediates and in the majority of the amino acids but unaltered pyridine nucleotide content both in leaves and during the progression of fruit ripening. Moreover, they displayed a generally accelerated development exhibiting early flowering, accelerated fruit ripening, and a markedly earlier onset of leaf senescence. In addition, transcript and selective hormone profiling of gibberellins and abscisic acid revealed changes only in the former coupled to changes in transcripts encoding enzymes of gibberellin biosynthesis. The data obtained are discussed in the context of the importance of this enzyme in both photosynthetic and respiratory metabolism as well as in programs of plant development connected to carbon-nitrogen interactions.

Details

show
hide
Language(s): eng - English
 Dates: 2012-07-042012
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000306919300011
DOI: 10.1105/tpc.112.099002
ISSN: 1532-298X (Electronic)1040-4651 (Linking)
URI: ://000306919300011http://www.plantcell.org/content/24/6/2328.full.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 24 (6) Sequence Number: - Start / End Page: 2328 - 2351 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X