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  Autoinducers act as biological timers in Vibrio harveyi

Anetzberger, C., Reiger, M., Fekete, A., Schell, U., Stambrau, N., Plener, L., et al. (2012). Autoinducers act as biological timers in Vibrio harveyi. PLoS One, 7(10), e48310. doi:10.1371/journal.pone.0048310.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-206E-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0018-03C1-B
Genre: Journal Article

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 Creators:
Anetzberger, C.1, Author
Reiger, M.1, Author
Fekete, A.1, Author
Schell, U.1, Author
Stambrau, N.1, Author
Plener, L.1, Author
Kopka, J.2, Author              
Schmitt-Kopplin, P.1, Author
Hilbi, H.1, Author
Jung, K.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

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 Abstract: Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1, AI-2 and CAI-1 are recognized by different receptors, but all information is channeled into the same signaling cascade, which controls a specific set of genes. Here we examine temporal variations of availability and concentration of the three autoinducers in V. harveyi, and monitor the phenotypes they regulate, from the early exponential to the stationary growth phase in liquid culture. Specifically, the exponential growth phase is characterized by an increase in AI-2 and the induction of bioluminescence, while HAI-1 and CAI-1 are undetectable prior to the late exponential growth phase. CAI-1 activity reaches its maximum upon entry into stationary phase, while molar concentrations of AI-2 and HAI-1 become approximately equal. Similarly, autoinducer-dependent exoproteolytic activity increases at the transition into stationary phase. These findings are reflected in temporal alterations in expression of the luxR gene that encodes the master regulator LuxR, and of four autoinducer-regulated genes during growth. Moreover, in vitro phosphorylation assays reveal a tight correlation between the HAI-1/AI-2 ratio as input and levels of receptor-mediated phosphorylation of LuxU as output. Our study supports a model in which the combinations of autoinducers available, rather than cell density per se, determine the timing of various processes in V. harveyi populations.

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Language(s): eng - English
 Dates: 2012-10-312012
 Publication Status: Published in print
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 7 (10) Sequence Number: - Start / End Page: e48310 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850