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  Evolutionary constraints on the plastid tRNA set decoding methionine and isoleucine

Alkatib, S., Fleischmann, T. T., Scharff, L. B., & Bock, R. (2012). Evolutionary constraints on the plastid tRNA set decoding methionine and isoleucine. Nucleic Acids Research, 40(14), 6713-6724. doi:10.1093/nar/gks350.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-207A-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-207B-C
Genre: Journal Article

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 Creators:
Alkatib, S.1, Author              
Fleischmann, T. T.1, Author              
Scharff, L. B.1, Author              
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: Chloroplasts/genetics *Codon *Evolution, Molecular Gene Deletion Gene Targeting *Genes, Chloroplast Isoleucine/*metabolism Methionine/*metabolism Phylogeny RNA, Transfer/chemistry/*genetics Tobacco/anatomy & histology/genetics/metabolism
 Abstract: The plastid (chloroplast) genomes of seed plants typically encode 30 tRNAs. Employing wobble and superwobble mechanisms, most codon boxes are read by only one or two tRNA species. The reduced set of plastid tRNAs follows the evolutionary trend of organellar genomes to shrink in size and coding capacity. A notable exception is the AUN codon box specifying methionine and isoleucine, which is decoded by four tRNA species in nearly all seed plants. However, three of these four tRNA genes were lost from the genomes of some parasitic plastid-containing lineages, possibly suggesting that less than four tRNA species could be sufficient to decode the triplets in the AUN box. To test this hypothesis, we have performed knockout experiments for the four AUN-decoding tRNAs in tobacco (Nicotiana tabacum) plastids. We find that all four tRNA genes are essential under both autotrophic and heterotrophic growth conditions, possibly suggesting tRNA import into plastids of parasitic plastid-bearing species. Phylogenetic analysis of the four plastid tRNA genes reveals striking conservation of all those bacterial features that are involved in discrimination between the different tRNA species containing CAU anticodons.

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Language(s): eng - English
 Dates: 2012-05-042012
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
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Title: Nucleic Acids Research
Source Genre: Journal
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Pages: - Volume / Issue: 40 (14) Sequence Number: - Start / End Page: 6713 - 6724 Identifier: -