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  Toward the storage metabolome: profiling the barley vacuole

Tohge, T., Ramos, M. S., Nunes-Nesi, A., Mutwil, M., Giavalisco, P., Steinhauser, D., et al. (2011). Toward the storage metabolome: profiling the barley vacuole. Plant Physiology, 157(3), 1469-1482. doi:10.​1104/​pp.​111.​185710.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-209F-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-065D-6
Genre: Journal Article

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 Creators:
Tohge, T.1, Author              
Ramos, M. S.2, Author
Nunes-Nesi, A.1, Author              
Mutwil, M.3, 4, Author              
Giavalisco, P.5, Author              
Steinhauser, D.6, 7, Author              
Schellenberg, M.2, Author
Willmitzer, L.6, Author              
Persson, S.3, Author              
Martinoia, E.2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              
3Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              
4Regulatory Networks, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753332              
5Experimental Systems Biology, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753342              
6Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
7Systems Metabolomics, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, Am Mühlenberg 1, 14476 Potsdam-Golm, DE, ou_1797285              

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Free keywords: Chromatography, Liquid Cluster Analysis Gas Chromatography-Mass Spectrometry Gene Regulatory Networks/genetics Hordeum/*metabolism Metabolic Networks and Pathways/genetics *Metabolome Metabolomics/*methods Protoplasts/metabolism Subcellular Fractions/metabolism Vacuoles/*metabolism
 Abstract: While recent years have witnessed dramatic advances in our capacity to identify and quantify an ever-increasing number of plant metabolites, our understanding of how metabolism is spatially regulated is still far from complete. In an attempt to partially address this question, we studied the storage metabolome of the barley (Hordeum vulgare) vacuole. For this purpose, we used highly purified vacuoles isolated by silicon oil centrifugation and compared their metabolome with that found in the mesophyll protoplast from which they were derived. Using a combination of gas chromatography-mass spectrometry and Fourier transform-mass spectrometry, we were able to detect 59 (primary) metabolites for which we know the exact chemical structure and a further 200 (secondary) metabolites for which we have strong predicted chemical formulae. Taken together, these metabolites comprise amino acids, organic acids, sugars, sugar alcohols, shikimate pathway intermediates, vitamins, phenylpropanoids, and flavonoids. Of the 259 putative metabolites, some 12 were found exclusively in the vacuole and 34 were found exclusively in the protoplast, while 213 were common in both samples. When analyzed on a quantitative basis, however, there is even more variance, with more than 60 of these compounds being present above the detection limit of our protocols. The combined data were also analyzed with respect to the tonoplast proteome in an attempt to infer specificities of the transporter proteins embedded in this membrane. Following comparison with recent observations made using nonaqueous fractionation of Arabidopsis (Arabidopsis thaliana), we discuss these data in the context of current models of metabolic compartmentation in plants.

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Language(s): eng - English
 Dates: 2011-09-292011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: 21949213
DOI: 10.​1104/​pp.​111.​185710
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: http://www.ncbi.nlm.nih.gov/pubmed/21949213
 Degree: -

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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 157 (3) Sequence Number: - Start / End Page: 1469 - 1482 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438