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  Transcription Factor-Dependent Chromatin Remodeling at Heat Shock and Copper-Responsive Promoters in Chlamydomonas reinhardtii

Strenkert, D., Schmollinger, S., Sommer, F., Schulz-Raffelt, M., & Schroda, M. (2011). Transcription Factor-Dependent Chromatin Remodeling at Heat Shock and Copper-Responsive Promoters in Chlamydomonas reinhardtii. Plant Cell, 23(6), 2285-2301. doi:10.1105/tpc.111.085266.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20B3-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20B4-C
Genre: Journal Article

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Strenkert-2011-Transcription Factor.pdf (Any fulltext), 2MB
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 Creators:
Strenkert, D.1, Author              
Schmollinger, S.1, Author              
Sommer, F.1, Author              
Schulz-Raffelt, M.1, Author              
Schroda, M.1, Author              
Affiliations:
1Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753312              

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Free keywords: histone-h3 lysine-4 methylation targeted recruitment nucleosome occupancy hsp70a promoter gene-expression domain protein culture cells genome-wide DNA-binding phd finger
 Abstract: How transcription factors affect chromatin structure to regulate gene expression in response to changes in environmental conditions is poorly understood in the green lineage. To shed light on this issue, we used chromatin immunoprecipitation and formaldehyde-assisted isolation of regulatory elements to investigate the chromatin structure at target genes of HSF1 and CRR1, key transcriptional regulators of the heat shock and copper starvation responses, respectively, in the unicellular green alga Chlamydomonas reinhardtii. Generally, we detected lower nucleosome occupancy, higher levels of histone H3/4 acetylation, and lower levels of histone H3 Lys 4 (H3K4) monomethylation at promoter regions of active genes compared with inactive promoters and transcribed and intergenic regions. Specifically, we find that activated HSF1 and CRR1 transcription factors mediate the acetylation of histones H3/4, nucleosome eviction, remodeling of the H3K4 mono-and dimethylation marks, and transcription initiation/elongation. By this, HSF1 and CRR1 quite individually remodel and activate target promoters that may be inactive and embedded into closed chromatin (HSP22F/CYC6) or weakly active and embedded into partially opened (CPX1) or completely opened chromatin (HSP70A/CRD1). We also observed HSF1-independent histone H3/4 deacetylation at the RBCS2 promoter after heat shock, suggesting interplay of specific and presumably more generally acting factors to adapt gene expression to the new requirements of a changing environment.

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Language(s): eng - English
 Dates: 2011-06-242011
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000293224200021
DOI: 10.1105/tpc.111.085266
URI: ://000293224200021 http://www.plantcell.org/content/23/6/2285.full.pdf
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Title: Plant Cell
Source Genre: Journal
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Pages: - Volume / Issue: 23 (6) Sequence Number: - Start / End Page: 2285 - 2301 Identifier: -