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  Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

Strenkert, D., Schmollinger, S., & Schroda, M. (2011). Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii. Plant Methods, 7, 35. doi:10.1186/1746-4811-7-35.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20B6-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20B7-6
Genre: Journal Article

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Strenkert, D.1, Author              
Schmollinger, S.1, Author              
Schroda, M.1, Author              
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1Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753312              

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Free keywords: chromatin immunoprecipitation chlamydomonas reinhardtii heat shock formaldehyde crosslinking real-time pcr nucleosome occupancy histone modification hsp70a rbcs2 cyc6 carbon-concentrating mechanism protein DNA interactions heat-shock gene formaldehyde acetylation regulator regions core
 Abstract: We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.

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Language(s): eng - English
 Dates: 2011-11-032011
 Publication Status: Published in print
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 Identifiers: DOI: 10.1186/1746-4811-7-35
ISSN: 1746-4811
URI: ://000297406800001 http://www.plantmethods.com/content/pdf/1746-4811-7-35.pdf
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Title: Plant Methods
Source Genre: Journal
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Pages: - Volume / Issue: 7 Sequence Number: - Start / End Page: 35 Identifier: -