English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
Add to Basket
 Local TagsRelease HistoryDetailsSummary
  Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii

Strenkert, D., Schmollinger, S., & Schroda, M. (2011). Protocol: methodology for chromatin immunoprecipitation (ChIP) in Chlamydomonas reinhardtii. Plant Methods, 7, 35. doi:10.1186/1746-4811-7-35.

Item is

 

show all hide all

Basic

show hide
Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-20B6-8 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0014-20B7-6
Genre: Journal Article

Files

show Files
hide Files
:
Strenkert-2011-Protocol_ methodolog.pdf (Any fulltext), 3MB
View   Save
File Permalink:
https://hdl.handle.net/11858/00-001M-0000-0014-20B8-4
Name:
Strenkert-2011-Protocol_ methodolog.pdf
Description:
-
OA-Status:
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
View
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Strenkert, D.1, Author           
Schmollinger, S.1, Author           
Schroda, M.1, Author           
Affiliations:
1Plant Molecular Chaperone Networks and Stress, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753312              

Content

show
hide
Free keywords: chromatin immunoprecipitation chlamydomonas reinhardtii heat shock formaldehyde crosslinking real-time pcr nucleosome occupancy histone modification hsp70a rbcs2 cyc6 carbon-concentrating mechanism protein DNA interactions heat-shock gene formaldehyde acetylation regulator regions core
 Abstract: We report on a detailed chromatin immunoprecipitation (ChIP) protocol for the unicellular green alga Chlamydomonas reinhardtii. The protocol is suitable for the analysis of nucleosome occupancy, histone modifications and transcription factor binding sites at the level of mononucleosomes for targeted and genome-wide studies. We describe the optimization of conditions for crosslinking, chromatin fragmentation and antibody titer determination and provide recommendations and an example for the normalization of ChIP results as determined by real-time PCR.

Details

show
hide
Language(s): eng - English
 Dates: 2011-11-032011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1186/1746-4811-7-35
ISSN: 1746-4811
URI: ://000297406800001 http://www.plantmethods.com/content/pdf/1746-4811-7-35.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Methods
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 Sequence Number: - Start / End Page: 35 Identifier: -