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  Live Cell Imaging Reveals Structural Associations between the Actin and Microtubule Cytoskeleton in Arabidopsis

Sampathkumar, A., Lindeboom, J. J., Debolt, S., Gutierrez, R., Ehrhardt, D. W., Ketelaar, T., et al. (2011). Live Cell Imaging Reveals Structural Associations between the Actin and Microtubule Cytoskeleton in Arabidopsis. Plant Cell, 23(6), 2302-2313. doi:10.1105/tpc.111.087940.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20E0-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-20E1-6
Genre: Journal Article

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 Creators:
Sampathkumar, A.1, Author              
Lindeboom, J. J.2, Author
Debolt, S.2, Author
Gutierrez, R.2, Author
Ehrhardt, D. W.2, Author
Ketelaar, T.2, Author
Persson, S.1, Author              
Affiliations:
1Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              
2External Organizations, ou_persistent22              

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Free keywords: cortical microtubules epidermal-cells dinitroaniline herbicide hair-cells f-actin organization interacts dynamics array microfilaments
 Abstract: In eukaryotic cells, the actin and microtubule (MT) cytoskeletal networks are dynamic structures that organize intracellular processes and facilitate their rapid reorganization. In plant cells, actin filaments (AFs) and MTs are essential for cell growth and morphogenesis. However, dynamic interactions between these two essential components in live cells have not been explored. Here, we use spinning-disc confocal microscopy to dissect interaction and cooperation between cortical AFs and MTs in Arabidopsis thaliana, utilizing fluorescent reporter constructs for both components. Quantitative analyses revealed altered AF dynamics associated with the positions and orientations of cortical MTs. Reorganization and reassembly of the AF array was dependent on the MTs following drug-induced depolymerization, whereby short AFs initially appeared colocalized with MTs, and displayed motility along MTs. We also observed that light-induced reorganization of MTs occurred in concert with changes in AF behavior. Our results indicate dynamic interaction between the cortical actin and MT cytoskeletons in interphase plant cells.

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Language(s): eng - English
 Dates: 2011-06-212011
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000293224200022
DOI: 10.1105/tpc.111.087940
ISSN: 1040-4651
URI: ://000293224200022 http://www.plantcell.org/content/23/6/2302.full.pdf
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Title: Plant Cell
Source Genre: Journal
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Pages: - Volume / Issue: 23 (6) Sequence Number: - Start / End Page: 2302 - 2313 Identifier: -