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  ATP Synthase Repression in Tobacco Restricts Photosynthetic Electron Transport, CO2 Assimilation, and Plant Growth by Overacidification of the Thylakoid Lumen

Rott, M., Martins, N. F., Thiele, W., Lein, W., Bock, R., Kraemer, D. M., et al. (2011). ATP Synthase Repression in Tobacco Restricts Photosynthetic Electron Transport, CO2 Assimilation, and Plant Growth by Overacidification of the Thylakoid Lumen. The Plant Cell, 23(1), 304-321. doi:10.1105/tpc.110.079111.

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 Creators:
Rott, M.1, Author              
Martins, N. F.1, Author              
Thiele, W.2, Author              
Lein, W.3, Author              
Bock, R.2, Author              
Kraemer, D. M.4, Author
Schöttler, M. A.1, Author              
Affiliations:
1Photosynthesis Research, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753323              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              
3Gene Function, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753333              
4External Organizations, ou_persistent22              

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Free keywords: cytochrome b(6)f complex transgenic tobacco gene-expression in-vivo chlamydomonas-reinhardtii arabidopsis-thaliana photosystem-ii redox state chloroplast plastocyanin
 Abstract: Tobacco (Nicotiana tabacum) plants strictly adjust the contents of both ATP synthase and cytochrome b(6)f complex to the metabolic demand for ATP and NADPH. While the cytochrome b(6)f complex catalyzes the rate-limiting step of photosynthetic electron flux and thereby controls assimilation, the functional significance of the ATP synthase adjustment is unknown. Here, we reduced ATP synthase accumulation by an antisense approach directed against the essential nuclear-encoded gamma-subunit (AtpC) and by the introduction of point mutations into the translation initiation codon of the plastid-encoded atpB gene (encoding the essential beta-subunit) via chloroplast transformation. Both strategies yielded transformants with ATP synthase contents ranging from 100 to <10% of wild-type levels. While the accumulation of the components of the linear electron transport chain was largely unaltered, linear electron flux was strongly inhibited due to decreased rates of plastoquinol reoxidation at the cytochrome b(6)f complex (photosynthetic control). Also, nonphotochemical quenching was triggered at very low light intensities, strongly reducing the quantum efficiency of CO2 fixation. We show evidence that this is due to an increased steady state proton motive force, resulting in strong lumen overacidification, which in turn represses photosynthesis due to photosynthetic control and dissipation of excitation energy in the antenna bed.

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Language(s): eng - English
 Dates: 2011-01-282011
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: ISI: ISI:000287860300023
DOI: 10.1105/tpc.110.079111
ISSN: 1040-4651
URI: ://000287860300023http://www.plantcell.org/content/23/1/304.full.pdf
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 23 (1) Sequence Number: - Start / End Page: 304 - 321 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X