English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis

Rautengarten, C., Ebert, B., Herter, T., Petzold, C. J., Ishii, T., Mukhopadhyay, A., et al. (2011). The Interconversion of UDP-Arabinopyranose and UDP-Arabinofuranose Is Indispensable for Plant Development in Arabidopsis. Plant Cell, 23(4), 1373-1390. doi:10.1105/tpc.111.083931.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2103-0 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2104-E
Genre: Journal Article

Files

show Files
hide Files
:
Rautengarten-2011-The Interconversion.pdf (Any fulltext), 2MB
Name:
Rautengarten-2011-The Interconversion.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Rautengarten, C.1, Author
Ebert, B.2, Author              
Herter, T.3, Author              
Petzold, C. J.1, Author
Ishii, T.1, Author
Mukhopadhyay, A.1, Author
Usadel, B.3, Author              
Scheller, H. V.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Biophysical Analysis, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753307              
3Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              

Content

show
hide
Free keywords: reversibly glycosylated polypeptide seed coat development rhamnogalacturonan-i arabinosyltransferase activity biochemical-characterization protein transglucosylase molecular-cloning complex-formation pectic arabinan biosynthesis
 Abstract: L-Ara, an important constituent of plant cell walls, is found predominantly in the furanose rather than in the thermodynamically more stable pyranose form. Nucleotide sugar mutases have been demonstrated to interconvert UDP-L-arabinopyranose (UDP-Arap) and UDP-L-arabinofuranose (UDP-Araf) in rice (Oryza sativa). These enzymes belong to a small gene family encoding the previously named Reversibly Glycosylated Proteins (RGPs). RGPs are plant-specific cytosolic proteins that tend to associate with the endomembrane system. In Arabidopsis thaliana, the RGP protein family consists of five closely related members. We characterized all five RGPs regarding their expression pattern and subcellular localizations in transgenic Arabidopsis plants. Enzymatic activity assays of recombinant proteins expressed in Escherichia coli identified three of the Arabidopsis RGP protein family members as UDP-L-Ara mutases that catalyze the formation of UDP-Araf from UDP-Arap. Coimmunoprecipitation and subsequent liquid chromatography-electrospray ionization-tandem mass spectrometry analysis revealed a distinct interaction network between RGPs in different Arabidopsis organs. Examination of cell wall polysaccharide preparations from RGP1 and RGP2 knockout mutants showed a significant reduction in total L-Ara content (12-31%) compared with wild-type plants. Concomitant downregulation of RGP1 and RGP2 expression results in plants almost completely deficient in cell wall-derived L-Ara and exhibiting severe developmental defects.

Details

show
hide
Language(s): eng - English
 Dates: 2011-04-082011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000291000500018
DOI: 10.1105/tpc.111.083931
ISSN: 1040-4651
URI: ://000291000500018 http://www.plantcell.org/content/23/4/1373.full.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Cell
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 23 (4) Sequence Number: - Start / End Page: 1373 - 1390 Identifier: -