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  Systems Biology of Tomato Fruit Development: Combined Transcript, Protein, and Metabolite Analysis of Tomato Transcription Factor (nor, rin) and Ethylene Receptor (Nr) Mutants Reveals Novel Regulatory Interactions

Osorio, S., Alba, R., Damasceno, C. M. B., Lopez-Casado, G., Lohse, M., Zanor, M. I., et al. (2011). Systems Biology of Tomato Fruit Development: Combined Transcript, Protein, and Metabolite Analysis of Tomato Transcription Factor (nor, rin) and Ethylene Receptor (Nr) Mutants Reveals Novel Regulatory Interactions. Plant Physiology, 157(1), 405-425. doi:10.1104/pp.111.175463.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2125-4 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-83BC-1
Genre: Journal Article

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 Creators:
Osorio, S.1, Author              
Alba, R.2, Author
Damasceno, C. M. B.2, Author
Lopez-Casado, G.2, Author
Lohse, M.3, Author              
Zanor, M. I.1, Author              
Tohge, T.1, Author              
Usadel, B.3, Author              
Rose, J. K. C.2, Author
Fei, Z. J.2, Author
Giovannoni, J. J.2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              
3Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              

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Free keywords: sample extraction techniques enhanced proteomic analysis inducible gene-expression cdna microarray data ripening-inhibitor signal-transduction never-ripe cell-walls carotenoid biosynthesis tissue specialization
 Abstract: Tomato (Solanum lycopersicum) is an established model to study fleshy fruit development and ripening. Tomato ripening is regulated independently and cooperatively by ethylene and transcription factors, including nonripening (NOR) and ripening-inhibitor (RIN). Mutations of NOR, RIN, and the ethylene receptor Never-ripe (Nr), which block ethylene perception and inhibit ripening, have proven to be great tools for advancing our understanding of the developmental programs regulating ripening. In this study, we present systems analysis of nor, rin, and Nr at the transcriptomic, proteomic, and metabolomic levels during development and ripening. Metabolic profiling marked shifts in the abundance of metabolites of primary metabolism, which lead to decreases in metabolic activity during ripening. When combined with transcriptomic and proteomic data, several aspects of the regulation of metabolism during ripening were revealed. First, correlations between the expression levels of a transcript and the abundance of its corresponding protein were infrequently observed during early ripening, suggesting that posttranscriptional regulatory mechanisms play an important role in these stages; however, this correlation was much greater in later stages. Second, we observed very strong correlation between ripening-associated transcripts and specific metabolite groups, such as organic acids, sugars, and cell wall-related metabolites, underlining the importance of these metabolic pathways during fruit ripening. These results further revealed multiple ethylene-associated events during tomato ripening, providing new insights into the molecular biology of ethylene-mediated ripening regulatory networks.

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Language(s): eng - English
 Dates: 2011-07-272011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000294491800031
DOI: 10.1104/pp.111.175463
ISSN: 0032-0889
URI: ://000294491800031http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3165888/pdf/405.pdf?tool=pmcentrez
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 157 (1) Sequence Number: - Start / End Page: 405 - 425 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438