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  Tissue- and Cell-Type Specific Transcriptome Profiling of Expanding Tomato Fruit Provides Insights into Metabolic and Regulatory Specialization and Cuticle Formation

Matas, A. J., Yeats, T. H., Buda, G. J., Zheng, Y., Chatterjee, S., Tohge, T., et al. (2011). Tissue- and Cell-Type Specific Transcriptome Profiling of Expanding Tomato Fruit Provides Insights into Metabolic and Regulatory Specialization and Cuticle Formation. The Plant Cell, 23(11), 3893-3910. doi:10.1105/tpc.111.091173.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2158-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-838D-B
Genre: Journal Article

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 Creators:
Matas, A. J.1, Author
Yeats, T. H.1, Author
Buda, G. J.1, Author
Zheng, Y.1, Author
Chatterjee, S.1, Author
Tohge, T.2, Author              
Ponnala, L.1, Author
Adato, A.1, Author
Aharoni, A.1, Author
Stark, R.1, Author
Fernie, A. R.2, Author              
Fei, Z.1, Author
Giovannoni, J. J.1, Author
Rose, J. K. C.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: laser-capture microdissection gene-expression abscission zone wall metabolism systems biology ripening fruit reveals arabidopsis epidermis genomics
 Abstract: Tomato (Solanum lycopersicum) is the primary model for the study of fleshy fruits, and research in this species has elucidated many aspects of fruit physiology, development, and metabolism. However, most of these studies have involved homogenization of the fruit pericarp, with its many constituent cell types. Here, we describe the coupling of pyrosequencing technology with laser capture microdissection to characterize the transcriptomes of the five principal tissues of the pericarp from tomato fruits (outer and inner epidermal layers, collenchyma, parenchyma, and vascular tissues) at their maximal growth phase. A total of 20,976 high-quality expressed unigenes were identified, of which more than half were ubiquitous in their expression, while others were cell type specific or showed distinct expression patterns in specific tissues. The data provide new insights into the spatial distribution of many classes of regulatory and structural genes, including those involved in energy metabolism, source-sink relationships, secondary metabolite production, cell wall biology, and cuticle biogenesis. Finally, patterns of similar gene expression between tissues led to the characterization of a cuticle on the inner surface of the pericarp, demonstrating the utility of this approach as a platform for biological discovery.

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Language(s): eng - English
 Dates: 2011-11-012011
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000298674200007
DOI: 10.1105/tpc.111.091173
ISSN: 1040-4651
URI: ://000298674200007http://www.plantcell.org/content/23/11/3893.full.pdf
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 23 (11) Sequence Number: - Start / End Page: 3893 - 3910 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X