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  NFX1-LIKE2 (NFXL2) Suppresses Abscisic Acid Accumulation and Stomatal Closure in Arabidopsis thaliana

Lisso, J., Schroeder, F., Fisahn, J., & Muessig, C. (2011). NFX1-LIKE2 (NFXL2) Suppresses Abscisic Acid Accumulation and Stomatal Closure in Arabidopsis thaliana. PLoS One, 6(11), e26982. doi:10.1371/journal.pone.0026982.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-216C-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-216D-6
Genre: Journal Article

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 Creators:
Lisso, J.1, Author              
Schroeder, F.1, Author              
Fisahn, J.2, Author              
Muessig, C.1, 3, Author              
Affiliations:
1Brassinosteroids, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753314              
2Biophysical Analysis, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753307              
3Developmental Physiology and Genomics, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753313              

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Free keywords: activated protein-kinases drought stress tolerance cell signal-transduction gene-expression transcription factors h2o2 accumulation hydrogen-peroxide oxidative stress water-stress aba
 Abstract: The NFX1-LIKE1 (NFXL1) and NFXL2 genes were identified as regulators of salt stress responses. The NFXL1 protein is a nuclear factor that positively affects adaptation to salt stress. The nfxl1-1 loss-of-function mutant displayed reduced survival rates under salt and high light stress. In contrast, the nfxl2-1 mutant, defective in the NFXL2 gene, and NFXL2-antisense plants exhibited enhanced survival under these conditions. We show here that the loss of NFXL2 function results in abscisic acid (ABA) overaccumulation, reduced stomatal conductance, and enhanced survival under drought stress. The nfxl2-1 mutant displayed reduced stomatal aperture under all conditions tested. Fusicoccin treatment, exposition to increasing light intensities, and supply of decreasing CO(2) concentrations demonstrated full opening capacity of nfxl2-1 stomata. Reduced stomatal opening presumably is a consequence of elevated ABA levels. Furthermore, seedling growth, root growth, and stomatal closure were hypersensitive to exogenous ABA. The enhanced ABA responses may contribute to the improved drought stress resistance of the mutant. Three NFXL2 splice variants were cloned and named NFXL2-78, NFXL2-97, and NFXL2-100 according to the molecular weight of the putative proteins. Translational fusions to the green fluorescent protein suggest nuclear localisation of the NFXL2 proteins. Stable expression of the NFXL2-78 splice variant in nfxl2-1 plants largely complemented the mutant phenotype. Our data show that NFXL2 controls ABA levels and suppresses ABA responses. NFXL2 may prevent unnecessary and costly stress adaptation under favourable conditions.

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Language(s): eng - English
 Dates: 2011-11-032011
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000297197000018
DOI: 10.1371/journal.pone.0026982
ISSN: 1932-6203
URI: ://000297197000018 http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0026982&representation=PDF
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 6 (11) Sequence Number: - Start / End Page: e26982 Identifier: -