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  The genetic architecture of branched-chain amino acid accumulation in tomato fruits

Kochevenko, A., & Fernie, A. R. (2011). The genetic architecture of branched-chain amino acid accumulation in tomato fruits. Journal of Experimental Botany, 62(11), 3895-3906. doi:10.1093/jxb/err091.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2197-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2198-4
Genre: Journal Article

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Kochevenko, A.1, Author              
Fernie, A. R.1, Author              
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1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: branched-chain amino acid introgression line metabolic regulation quantitative trait analysis reverse genetics tomato fruit metabolism quantitative trait loci wild-species tomato lycopersicon-esculentum spinach-chloroplasts arabidopsis-thaliana valine biosynthesis metabolic pathways fatty-acids leucine catabolism
 Abstract: Previous studies of the genetic architecture of fruit metabolic composition have allowed us to identify four strongly conserved co-ordinate quantitative trait loci (QTL) for the branched-chain amino acids (BCAAs). This study has been extended here to encompass the other 23 enzymes described to be involved in the pathways of BCAA synthesis and degradation. On coarse mapping the chromosomal location of these enzymes, it was possible to define the map position of 24 genes. Of these genes eight co-localized, or mapped close to BCAA QTL including those encoding ketol-acid reductoisomerase (KARI), dihydroxy-acid dehydratase (DHAD), and isopropylmalate dehydratase (IPMD). Quantitative evaluation of the expression levels of these genes revealed that the S. pennellii allele of IPMD demonstrated changes in the expression level of this gene, whereas those of KARI and DHAD were invariant across the genotypes. Whilst the antisense inhibition of IPMD resulted in increased BCAA, the antisense inhibition of neither KARI nor DHAD produced a clear effect in fruit BCAA contents. The results are discussed both with respect to the roles of these specific enzymes within plant amino acid metabolism and within the context of current understanding of the regulation of plant branched-chain amino acid metabolism.

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Language(s): eng - English
 Dates: 2011-03-242011
 Publication Status: Published in print
 Pages: -
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 Identifiers: ISI: ISI:000292838700017
DOI: 10.1093/jxb/err091
ISSN: 0022-0957
URI: ://000292838700017 http://jxb.oxfordjournals.org/content/62/11/3895.full.pdf
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Title: Journal of Experimental Botany
Source Genre: Journal
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Pages: - Volume / Issue: 62 (11) Sequence Number: - Start / End Page: 3895 - 3906 Identifier: -