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  A Deficiency in the Flavoprotein of Arabidopsis Mitochondrial Complex II Results in Elevated Photosynthesis and Better Growth in Nitrogen-Limiting Conditions

Fuentes, D., Meneses, M., Nunes-Nesi, A., Araujo, W., Tapia, R., Gomez, I., et al. (2011). A Deficiency in the Flavoprotein of Arabidopsis Mitochondrial Complex II Results in Elevated Photosynthesis and Better Growth in Nitrogen-Limiting Conditions. Plant Physiology, 157(3), 1114-1127. doi:10.1104/pp.111.183939.

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 Creators:
Fuentes, D.1, Author
Meneses, M.1, Author
Nunes-Nesi, A.2, Author              
Araujo, W.1, Author
Tapia, R.1, Author
Gomez, I.1, Author
Holuigue, L.1, Author
Gutierrez, R. A.1, Author
Fernie, A. R.2, Author              
Jordana, X.1, Author
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1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: iron-sulfur subunit succinate-dehydrogenase carbon-dioxide nuclear gene enhances photosynthesis nitrate assimilation nicotiana-sylvestris illuminated leaves reduced expression respiratory-chain
 Abstract: Mitochondrial complex II (succinate dehydrogenase [SDH]) plays roles both in the tricarboxylic acid cycle and the respiratory electron transport chain. In Arabidopsis thaliana its flavoprotein subunit is encoded by two nuclear genes, SDH1-1 and SDH1-2. Here we characterize heterozygous SDH1-1/sdh1-1 mutant plants displaying a 30% reduction in SDH activity as well as partially silenced plants obtained by RNA interference. We found that these plants displayed significantly higher CO(2) assimilation rates and enhanced growth than wild type plants. There was a strong correlation between CO(2) assimilation and stomatal conductance and both mutant and silenced plants displayed increased stomatal aperture and density. By contrast no significant differences were found for dark respiration, chloroplastic electron transport rate, CO(2) uptake at saturating concentrations of CO(2) or biochemical parameters such as the maximum rates of carboxylation by Rubisco and of photosynthetic electron transport. Thus, photosynthesis is enhanced in SDH deficient plants by a mechanism involving a specific effect on stomatal function which results in improved CO(2) uptake. Metabolic and transcript profiling revealed that mild deficiency in SDH results in limited effects on metabolism and gene expression, and data suggest that decreases observed in the levels of some amino acids were due to a higher flux to proteins and other nitrogen-containing compounds to support increased growth. Strikingly, SDH1-1/sdh1-1 seedlings grew considerably better in nitrogen-limiting conditions. Thus, a subtle metabolic alteration may lead to changes in important functions such as stomatal function and nitrogen assimilation.

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Language(s): eng - English
 Dates: 2011-09-172011
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1104/pp.111.183939
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000296722300012http://www.plantphysiol.org/content/157/3/1114.full.pdf
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 157 (3) Sequence Number: - Start / End Page: 1114 - 1127 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438