English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Fondevilla, S., Kuester, H., Krajinski, F., Cubero, J. I., & Rubiales, D. (2011). Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology. BMC Genomics, 12, 28. doi:10.1186/1471-2164-12-28.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2202-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2203-8
Genre: Journal Article

Files

show Files
hide Files
:
Fondevilla-2011-Identification of ge.pdf (Any fulltext), 386KB
Name:
Fondevilla-2011-Identification of ge.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Fondevilla, S.1, Author
Kuester, H.1, Author
Krajinski, F.2, Author              
Cubero, J. I.1, Author
Rubiales, D.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Plant-Microbe Interactions, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753317              

Content

show
hide
Free keywords: quantitative trait loci pisum-sativum l. plant-disease resistance ascochyta blight resistance legume medicago-truncatula orobanche-crenata field pea abc transporters seed infection cell-walls
 Abstract: Background: Ascochyta blight, caused by Mycosphaerella pinodes is one of the most important pea pathogens. However, little is known about the genes and mechanisms of resistance acting against M. pinodes in pea. Resistance identified so far to this pathogen is incomplete, polygenic and scarce in pea, being most common in Pisum relatives. The identification of the genes underlying resistance would increase our knowledge about M. pinodes-pea interaction and would facilitate the introgression of resistance into pea varieties. In the present study differentially expressed genes in the resistant P. sativum ssp. syriacum accession P665 comparing to the susceptible pea cv. Messire after inoculation with M. pinodes have been identified using a M. truncatula microarray. Results: Of the 16,470 sequences analysed, 346 were differentially regulated. Differentially regulated genes belonged to almost all functional categories and included genes involved in defense such as genes involved in cell wall reinforcement, phenylpropanoid and phytoalexins metabolism, pathogenesis-related (PR) proteins and detoxification processes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced suggesting that the response to M. pinodes in pea is regulated via JA and ET pathways. Expression levels of ten differentially regulated genes were validated in inoculated and control plants using qRT-PCR showing that the P665 accession shows constitutively an increased expression of the defense related genes as peroxidases, disease resistance response protein 39 (DRR230-b), glutathione S-transferase (GST) and 6a-hydroxymaackiain methyltransferase. Conclusions: Through this study a global view of genes expressed during resistance to M. pinodes has been obtained, giving relevant information about the mechanisms and pathways conferring resistance to this important disease. In addition, the M. truncatula microarray represents an efficient tool to identify candidate genes controlling resistance to M. pinodes in pea.

Details

show
hide
Language(s): eng - English
 Dates: 2011-01-132011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000286609300001
DOI: 10.1186/1471-2164-12-28
ISSN: 1471-2164
URI: ://000286609300001 http://www.biomedcentral.com/content/pdf/1471-2164-12-28.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Genomics
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 12 Sequence Number: - Start / End Page: 28 Identifier: -