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  Targeted Enhancement of Glutamate-to-gamma-Aminobutyrate Conversion in Arabidopsis Seeds Affects Carbon-Nitrogen Balance and Storage Reserves in a Development-Dependent Manner

Fait, A., Nunes Nesi, A., Angelovici, R., Lehmann, M., Pham, P. A., Song, L., et al. (2011). Targeted Enhancement of Glutamate-to-gamma-Aminobutyrate Conversion in Arabidopsis Seeds Affects Carbon-Nitrogen Balance and Storage Reserves in a Development-Dependent Manner. Plant Physiology, 157(3), 1026-1042. doi:10.1104/pp.111.179986.

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Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2216-D Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-839B-B
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Fait-2011-Targeted Enhancement.pdf (beliebiger Volltext), 2MB
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 Urheber:
Fait, A.1, Autor              
Nunes Nesi, A.2, Autor
Angelovici, R.2, Autor
Lehmann, M.1, Autor              
Pham, P. A.1, Autor              
Song, L.2, Autor
Haslam, R. P.2, Autor
Napier, J. A.2, Autor
Galili, G.2, Autor
Fernie, A. R.1, Autor              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              

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Schlagwörter: calmodulin-binding domain developing soybean seeds fatty-acid synthesis amino-acids biochemical-characterization gas-chromatography metabolic networks fruit-development lysine synthesis brassica-napus
 Zusammenfassung: In seeds, glutamate decarboxylase (GAD) operates at the metabolic nexus between carbon and nitrogen metabolism by catalysing the unidirectional decarboxylation of Glu to form gamma-aminobutyric acid (GABA). To elucidate the regulatory role of GAD in seed development, we generated Arabidopsis thaliana transgenic plants expressing a truncated GAD from Petunia hybrida missing the C-terminal regulatory Ca2+-calmodulin (CaM) binding domain, under the transcriptional regulation of the seed maturation specific phaseolin promoter. Dry seeds of the transgenic plants accumulated considerable amounts of GABA, and during desiccation the content of several amino acids increased, though not Glu or Pro. Dry transgenic seeds had higher protein content than the wild-type seeds, but lower amounts of the intermediates of glycolysis, glycerol and malate. The total fatty acid (TFA) content of the transgenic seeds was 50% lower than in that of the wild-type, while acyl CoAs accumulated in the transgenic seeds. Labeling experiments revealed altered levels of respiration in the transgenic seeds, and fractionation studies indicated reduced incorporation of label in the sugar and lipid fractions extracted from transgenic seeds. Comparative transcript profiling of the dry seeds supported the metabolic data. Cellular processes upregulated at the transcript level included the tricarboxylic acid cycle, fatty acid elongation, the shikimate pathway and Trp metabolism, N-C remobilization and programmed cell death. Genes involved in the regulation of germination were similarly upregulated. Taken together these results indicate that the GAD-mediated conversion of Glu to GABA during seed development plays an important role in balancing carbon and nitrogen metabolism and in storage reserve accumulation.

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Sprache(n): eng - Englisch
 Datum: 2011-09-172011
 Publikationsstatus: Im Druck publiziert
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Titel: Plant Physiology
  Andere : Plant Physiol.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: Bethesda, Md. : American Society of Plant Biologists
Seiten: - Band / Heft: 157 (3) Artikelnummer: - Start- / Endseite: 1026 - 1042 Identifikator: ISSN: 0032-0889
CoNE: /journals/resource/991042744294438