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  Subfunctionalization of Cellulose Synthases in Seed Coat Epidermal Cells Mediates Secondary Radial Wall Synthesis and Mucilage Attachment

DeBolt, S., Mendu, V., Griffiths, J. S., Persson, S., Stork, J., Downie, A. B., et al. (2011). Subfunctionalization of Cellulose Synthases in Seed Coat Epidermal Cells Mediates Secondary Radial Wall Synthesis and Mucilage Attachment. Plant Physiology, 157(1), 441-453. doi:10.1104/pp.111.179069.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-223D-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-223E-6
Genre: Journal Article

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 Creators:
DeBolt, S.1, Author
Mendu, V.1, Author
Griffiths, J. S.1, Author
Persson, S.2, Author              
Stork, J.1, Author
Downie, A. B.1, Author
Voiniciuc, C.1, Author
Haughn, G. W.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

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Free keywords: arabidopsis-thaliana electron-microscopy flowering plants calcofluor white differentiation pectin morphogenesis complexes encodes galactosidase
 Abstract: Arabidopsis (Arabidopsis thaliana) epidermal seed coat cells follow a complex developmental program where, following fertilization, cells of the ovule outer integument differentiate into a unique cell type. Two hallmarks of these cells are the production of a doughnut-shaped apoplastic pocket filled with pectinaceous mucilage and the columella, a thick secondary cell wall. Cellulose is thought to be a key component of both these secondary cell wall processes. Here, we investigated the role of cellulose synthase (CESA) subunits CESA2, CESA5, and CESA9 in the seed coat epidermis. We characterized the roles of these CESA proteins in the seed coat by analyzing cell wall composition and morphology in cesa mutant lines. Mutations in any one of these three genes resulted in lower cellulose content, a loss of cell shape uniformity, and reduced radial wall integrity. In addition, we found that attachment of the mucilage halo to the parent seed following extrusion is maintained by cellulose-based connections requiring CESA5. Hence, we show that cellulose fulfills an adhesion role between the extracellular mucilage matrix and the parent cell in seed coat epidermal cells. We propose that mucilage remains attached to the seed coat through interactions between components in the seed mucilage and cellulose. Our data suggest that CESA2 and CESA9 serve in radial wall reinforcement, as does CESA5, but CESA5 also functions in mucilage biosynthesis. These data suggest unique roles for different CESA subunits in one cell type and illustrate a complex role for cellulose biosynthesis in plant developmental biology.

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Language(s): eng - English
 Dates: 2011-07-122011
 Publication Status: Published in print
 Pages: -
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 Identifiers: ISI: ISI:000294491800033
DOI: 10.1104/pp.111.179069
ISSN: 0032-0889
URI: ://000294491800033 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3165890/pdf/441.pdf?tool=pmcentrez
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Title: Plant Physiology
Source Genre: Journal
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Pages: - Volume / Issue: 157 (1) Sequence Number: - Start / End Page: 441 - 453 Identifier: -