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  Overexpression of Sinapine Esterase BnSCE3 in Oilseed Rape Seeds Triggers Global Changes in Seed Metabolism

Clauss, K., von Roepenack-Lahaye, E., Boettcher, C., Roth, M. R., Welti, R., Erban, A., et al. (2011). Overexpression of Sinapine Esterase BnSCE3 in Oilseed Rape Seeds Triggers Global Changes in Seed Metabolism. Plant Physiology, 155(3), 1127-1145. doi:10.1104/pp.110.169821.

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 Creators:
Clauss, K.1, Author
von Roepenack-Lahaye, E.1, Author
Boettcher, C.1, Author
Roth, M. R.1, Author
Welti, R.1, Author
Erban, A.2, Author              
Kopka, J.2, Author              
Scheel, D.1, Author
Milkowski, C.1, Author
Strack, D.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Applied Metabolome Analysis, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753338              

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Free keywords: raphanus-sativus l brassica-napus lignin biosynthesis mass-spectrometry udp-glucose phenylpropanoid metabolism acid glucosyltransferase quantitative-analysis arabidopsis-thaliana enzymatic-synthesis
 Abstract: Sinapine (O-sinapoylcholine) is the predominant phenolic compound in a complex group of sinapate esters in seeds of oilseed rape (Brassica napus). Sinapine has antinutritive activity and prevents the use of seed protein for food and feed. A strategy was developed to lower its content in seeds by expressing an enzyme that hydrolyzes sinapine in developing rape seeds. During early stages of seedling development, a sinapine esterase (BnSCE3) hydrolyzes sinapine, releasing choline and sinapate. A portion of choline enters the phospholipid metabolism, and sinapate is routed via 1-O-sinapoyl-b-glucose into sinapoylmalate. Transgenic oilseed rape lines were generated expressing BnSCE3 under the control of a seed-specific promoter. Two distinct single-copy transgene insertion lines were isolated and propagated to generate homozygous lines, which were subjected to comprehensive phenotyping. Sinapine levels of transgenic seeds were less than 5% of wild-type levels, whereas choline levels were increased. Weight, size, and water content of transgenic seeds were significantly higher than those of wild-type seeds. Seed quality parameters, such as fiber and glucosinolate levels, and agronomically important traits, such as oil and protein contents, differed only slightly, except that amounts of hemicellulose and cellulose were about 30% higher in transgenic compared with wild-type seeds. Electron microscopic examination revealed that a fraction of the transgenic seeds had morphological alterations, characterized by large cavities near the embryonic tissue. Transgenic seedlings were larger than wild-type seedlings, and young seedlings exhibited longer hypocotyls. Examination of metabolic profiles of transgenic seeds indicated that besides suppression of sinapine accumulation, there were other dramatic differences in primary and secondary metabolism. Mapping of these changes onto metabolic pathways revealed global effects of the transgenic BnSCE3 expression on seed metabolism.

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Language(s): eng - English
 Dates: 2011-01-192011
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000287843800008
DOI: 10.1104/pp.110.169821
ISSN: 0032-0889
URI: ://000287843800008http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3046574/pdf/1127.pdf?tool=pmcentrez
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 155 (3) Sequence Number: - Start / End Page: 1127 - 1145 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438