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  Malate Plays a Crucial Role in Starch Metabolism, Ripening, and Soluble Solid Content of Tomato Fruit and Affects Postharvest Softening

Centeno, D. C., Osorio, S., Nunes-Nesi, A., Bertolo, A. L. F., Carneiro, R. T., Araujo, W. L., et al. (2011). Malate Plays a Crucial Role in Starch Metabolism, Ripening, and Soluble Solid Content of Tomato Fruit and Affects Postharvest Softening. The Plant Cell, 23(1), 162-184. doi:10.1105/tpc.109.072231.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2252-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-8401-E
Genre: Journal Article

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Centeno, D. C.1, Author              
Osorio, S.1, Author              
Nunes-Nesi, A.1, Author              
Bertolo, A. L. F.2, Author
Carneiro, R. T.2, Author
Araujo, W. L.1, Author              
Steinhauser, M.-C.3, Author              
Michalska, J.3, Author              
Rohrmann, J.1, Author              
Geigenberger, P.4, Author              
Oliver, S. N.4, Author              
Stitt, M.3, Author              
Carrari, F.1, Author              
Rose, J. K. C.2, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2External Organizations, ou_persistent22              
3System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
4Storage Carbohydrate Metabolism, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753336              

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Free keywords: adp-glucose pyrophosphorylase growing potato-tubers adenosine 5'-diphosphoglucose pyrophosphorylase cell-wall metabolism gene-expression carotenoid biosynthesis carbohydrate-metabolism catalytic-properties solanum-tuberosum malic enzyme
 Abstract: Despite the fact that the organic acid content of a fruit is regarded as one of its most commercially important quality traits when assessed by the consumer, relatively little is known concerning the physiological importance of organic acid metabolism for the fruit itself. Here, we evaluate the effect of modifying malate metabolism in a fruit-specific manner, by reduction of the activities of either mitochondrial malate dehydrogenase or fumarase, via targeted antisense approaches in tomato (Solanum lycopersicum). While these genetic perturbations had relatively little effect on the total fruit yield, they had dramatic consequences for fruit metabolism, as well as unanticipated changes in postharvest shelf life and susceptibility to bacterial infection. Detailed characterization suggested that the rate of ripening was essentially unaltered but that lines containing higher malate were characterized by lower levels of transitory starch and a lower soluble sugars content at harvest, whereas those with lower malate contained higher levels of these carbohydrates. Analysis of the activation state of ADP-glucose pyrophosphorylase revealed that it correlated with the accumulation of transitory starch. Taken together with the altered activation state of the plastidial malate dehydrogenase and the modified pigment biosynthesis of the transgenic lines, these results suggest that the phenotypes are due to an altered cellular redox status. The combined data reveal the importance of malate metabolism in tomato fruit metabolism and development and confirm the importance of transitory starch in the determination of agronomic yield in this species.

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Language(s): eng - English
 Dates: 2011-01-142011
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000287860300015
DOI: 10.1105/tpc.109.072231
ISSN: 1040-4651
URI: ://000287860300015http://www.plantcell.org/content/23/1/162.full.pdf
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 23 (1) Sequence Number: - Start / End Page: 162 - 184 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X