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  Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes

Bazzini, A. A., Manacorda, C. A., Tohge, T., Conti, G., Rodriguez, M. C., Nunes-Nesi, A., et al. (2011). Metabolic and miRNA Profiling of TMV Infected Plants Reveals Biphasic Temporal Changes. PLoS One, 6(12), e28466. doi:10.1371/journal.pone.0028466.

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 Creators:
Bazzini, A. A.1, Author
Manacorda, C. A.1, Author
Tohge, T.2, Author              
Conti, G.1, Author
Rodriguez, M. C.1, Author
Nunes-Nesi, A.2, Author              
Villanueva, S.1, Author
Fernie, A. R.2, Author              
Carrari, F.2, Author              
Asurmendi, S.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: tobacco-mosaic-virus systemic acquired-resistance protein-mediated resistance rna silencing suppressors arabidopsis-thaliana gene-expression transgenic tobacco disease resistance gas-chromatography nicotiana-tabacum
 Abstract: Plant viral infections induce changes including gene expression and metabolic components. Identification of metabolites and microRNAs (miRNAs) differing in abundance along infection may provide a broad view of the pathways involved in signaling and defense that orchestrate and execute the response in plant-pathogen interactions. We used a systemic approach by applying both liquid and gas chromatography coupled to mass spectrometry to determine the relative level of metabolites across the viral infection, together with a miRs profiling using a micro-array based procedure. Systemic changes in metabolites were characterized by a biphasic response after infection. The first phase, detected at one dpi, evidenced the action of a systemic signal since no virus was detected systemically. Several of the metabolites increased at this stage were hormone-related. miRs profiling after infection also revealed a biphasic alteration, showing miRs alteration at 5 dpi where no virus was detected systemically and a late phase correlating with virus accumulation. Correlation analyses revealed a massive increase in the density of correlation networks after infection indicating a complex reprogramming of the regulatory pathways, either in response to the plant defense mechanism or to the virus infection itself. Our data propose the involvement of a systemic signaling on early miRs alteration.

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Language(s): eng - English
 Dates: 2011-12-122011
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000298366600019
DOI: 10.1371/journal.pone.0028466
ISSN: 1932-6203
URI: ://000298366600019http://www.plosone.org/article/fetchObjectAttachment.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0028466&representation=PDF
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: - Volume / Issue: 6 (12) Sequence Number: - Start / End Page: e28466 Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850