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  ORS1, an H(2)O(2)-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thaliana

Balazadeh, S., Kwasniewski, M., Caldana, C., Mehrnia, M., Zanor, M. I., Xue, G. P., et al. (2011). ORS1, an H(2)O(2)-Responsive NAC Transcription Factor, Controls Senescence in Arabidopsis thaliana. Molecular Plant, 4(2), 346-360. doi:10.1093/mp/ssq080.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2277-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-77E2-1
Genre: Journal Article

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 Creators:
Balazadeh, S.1, Author              
Kwasniewski, M.2, Author              
Caldana, C.3, Author              
Mehrnia, M.4, Author              
Zanor, M. I.2, Author              
Xue, G. P.5, Author
Mueller-Roeber, B.2, Author              
Affiliations:
1Transcription Factors and Gene Regulatory Networks, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753316              
2Plant Signalling, Cooperative Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753311              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
4Molecular Genomics, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753328              
5External Organizations, ou_persistent22              

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Free keywords: nac transcription factor leaf senescence gene expression gene regulatory network hydrogen peroxide leaf senescence gene-expression signaling pathways reactive oxygen oryza-sativa cell-death stress specificity database suggests
 Abstract: We report here that ORS1, a previously uncharacterized member of the NAC transcription factor family, controls leaf senescence in Arabidopsis thaliana. Overexpression of ORS1 accelerates senescence in transgenic plants, whereas its inhibition delays it. Genes acting downstream of ORS1 were identified by global expression analysis using transgenic plants producing dexamethasone-inducible ORS1-GR fusion protein. Of the 42 up-regulated genes, 30 (similar to 70%) were previously shown to be up-regulated during age-dependent senescence. We also observed that 32 (similar to 76%) of the ORS1-dependent genes were induced by long-term (4 d), but not short-term (6 h) salinity stress (150 mM NaCl). Furthermore, expression of 16 and 24 genes, respectively, was induced after 1 and 5 h of treatment with hydrogen peroxide (H(2)O(2)), a reactive oxygen species known to accumulate during salinity stress. ORS1 itself was found to be rapidly and strongly induced by H(2)O(2) treatment in both leaves and roots. Using in vitro binding site selection, we determined the preferred binding motif of ORS1 and found it to be present in half of the ORS1-dependent genes. ORS1 is a paralog of ORE1/ANAC092/AtNAC2, a previously reported regulator of leaf senescence. Phylogenetic footprinting revealed evolutionary conservation of the ORS1 and ORE1 promoter sequences in different Brassicaceae species, indicating strong positive selection acting on both genes. We conclude that ORS1, similarly to ORE1, triggers expression of senescence-associated genes through a regulatory network that may involve cross-talk with salt- and H(2)O(2)-dependent signaling pathways.

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Language(s): eng - English
 Dates: 2011-02-082011
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000288802100014
DOI: 10.1093/mp/ssq080
ISSN: 1674-2052
URI: ://000288802100014http://mplant.oxfordjournals.org/content/4/2/346.full.pdf
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Title: Molecular Plant
Source Genre: Journal
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Pages: - Volume / Issue: 4 (2) Sequence Number: - Start / End Page: 346 - 360 Identifier: -