English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Antisense Inhibition of the Iron-Sulphur Subunit of Succinate Dehydrogenase Enhances Photosynthesis and Growth in Tomato via an Organic Acid-Mediated Effect on Stomatal Aperture

Araujo, W. L., Nunes-Nesi, A., Osorio, S., Usadel, B., Fuentes, D., Nagy, R., et al. (2011). Antisense Inhibition of the Iron-Sulphur Subunit of Succinate Dehydrogenase Enhances Photosynthesis and Growth in Tomato via an Organic Acid-Mediated Effect on Stomatal Aperture. The Plant Cell, 23(2), 600-627. doi:10.1105/tpc.110.081224.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2284-8 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0019-83F7-E
Genre: Journal Article

Files

show Files
hide Files
:
Araujo-2011-Antisense Inhibition.pdf (Any fulltext), 3MB
Name:
Araujo-2011-Antisense Inhibition.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Araujo, W. L.1, Author              
Nunes-Nesi, A.1, Author              
Osorio, S.1, Author              
Usadel, B.2, Author              
Fuentes, D.3, Author
Nagy, R.3, Author
Balbo, I.1, Author              
Lehmann, M.1, Author              
Studart-Witkowski, C.1, Author              
Tohge, T.1, Author              
Martinoia, E.3, Author
Jordana, X.3, Author
DaMatta, F. M.3, Author
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2Integrative Carbon Biology, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753329              
3External Organizations, ou_persistent22              

Content

show
hide
Free keywords: mitochondrial-complex-ii arabidopsis guard-cells ribulose-1,5-bisphosphate carboxylase-oxygenase electron-transport chain cdna microarray data vicia-faba l abscisic-acid ion channels respiratory-chain ubiquinone oxidoreductase
 Abstract: Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the Sl SDH2-2 gene encoding the iron sulfur subunit of the succinate dehydrogenase protein complex in the antisense orientation under the control of the 35S promoter exhibit an enhanced rate of photosynthesis. The rate of the tricarboxylic acid (TCA) cycle was reduced in these transformants, and there were changes in the levels of metabolites associated with the TCA cycle. Furthermore, in comparison to wild-type plants, carbon dioxide assimilation was enhanced by up to 25% in the transgenic plants under ambient conditions, and mature plants were characterized by an increased biomass. Analysis of additional photosynthetic parameters revealed that the rate of transpiration and stomatal conductance were markedly elevated in the transgenic plants. The transformants displayed a strongly enhanced assimilation rate under both ambient and suboptimal environmental conditions, as well as an elevated maximal stomatal aperture. By contrast, when the Sl SDH2-2 gene was repressed by antisense RNA in a guard cell-specific manner, changes in neither stomatal aperture nor photosynthesis were observed. The data obtained are discussed in the context of the role of TCA cycle intermediates both generally with respect to photosynthetic metabolism and specifically with respect to their role in the regulation of stomatal aperture.

Details

show
hide
Language(s): eng - English
 Dates: 2011-02-092011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000288801000014
DOI: 10.1105/tpc.110.081224
ISSN: 1040-4651
URI: ://000288801000014http://www.plantcell.org/content/23/2/600.full.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 23 (2) Sequence Number: - Start / End Page: 600 - 627 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X