English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Genetic dissection of vitamin E biosynthesis in tomato

Almeida, J., Quadrana, L., Asis, R., Setta, N., de Godoy, F., Bermudez, L., et al. (2011). Genetic dissection of vitamin E biosynthesis in tomato. Journal of Experimental Botany, 62(11), 3781-3798. doi:10.1093/jxb/err055.

Item is

Files

show Files
hide Files
:
Almeida-2011-Genetic dissection o.pdf (Any fulltext), 884KB
Name:
Almeida-2011-Genetic dissection o.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Almeida, J.1, Author
Quadrana, L.1, Author
Asis, R.1, Author
Setta, N.1, Author
de Godoy, F.1, Author
Bermudez, L.1, Author
Otaiza, S. N.1, Author
da Silva, J. V. C.1, Author
Fernie, A. R.2, Author              
Carrari, F.2, Author              
Rossi, M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: fruit metabolism solanum pennellii tocopherol tomato vitamin e aromatic-amino-acids 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase geranyl diphosphate synthase multiple sequence alignment arabidopsis-thaliana subcellular compartmentation tocopherol biosynthesis isoprenoid biosynthesis transgenic tobacco shikimate pathway
 Abstract: Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for alpha-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (alpha, beta, gamma, and delta) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.

Details

show
hide
Language(s): eng - English
 Dates: 2011-04-282011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000292838700008
DOI: 10.1093/jxb/err055
ISSN: 1460-2431 (Electronic)0022-0957 (Linking)
URI: ://000292838700008http://jxb.oxfordjournals.org/content/62/11/3781.full.pdf
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Experimental Botany
  Other : J. Exp. Bot
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Oxford : Oxford University Press [etc.]
Pages: - Volume / Issue: 62 (11) Sequence Number: - Start / End Page: 3781 - 3798 Identifier: ISSN: 0022-0957
CoNE: https://pure.mpg.de/cone/journals/resource/954925413883