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  Genetic Analysis of Central Carbon Metabolism Unveils an Amino Acid Substitution That Alters Maize NAD-Dependent Isocitrate Dehydrogenase Activity

Zhang, N. Y., Gur, A., Gibon, Y., Sulpice, R., Flint-Garcia, S., McMullen, M. D., et al. (2010). Genetic Analysis of Central Carbon Metabolism Unveils an Amino Acid Substitution That Alters Maize NAD-Dependent Isocitrate Dehydrogenase Activity. PLoS One, 5(4), e9991. doi:10.1371/Journal.Pone.0009991.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-229D-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-229E-0
Genre: Journal Article

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 Creators:
Zhang, N. Y.1, Author
Gur, A.1, Author
Gibon, Y.2, Author              
Sulpice, R.2, Author              
Flint-Garcia, S.1, Author
McMullen, M. D.1, Author
Stitt, M.2, Author              
Buckler, E. S.1, Author
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1External Organizations, ou_persistent22              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

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Free keywords: nitrogen use efficiency mays ssp parviglumis linkage disequilibrium enzyme-activities drosophila-melanogaster alcohol-dehydrogenase candidate genes higher-plants association arabidopsis
 Abstract: Background: Central carbon metabolism (CCM) is a fundamental component of life. The participating genes and enzymes are thought to be structurally and functionally conserved across and within species. Association mapping utilizes a rich history of mutation and recombination to achieve high resolution mapping. Therefore, applying association mapping in maize (Zea mays ssp. mays), the most diverse model crop species, to study the genetics of CCM is a particularly attractive system. Methodology/Principal Findings: We used a maize diversity panel to test the CCM functional conservation. We found heritable variation in enzyme activity for every enzyme tested. One of these enzymes was the NAD-dependent isocitrate dehydrogenase (IDH, E.C. 1.1.1.41), in which we identified a novel amino-acid substitution in a phylogenetically conserved site. Using candidate gene association mapping, we identified that this non-synonymous polymorphism was associated with IDH activity variation. The proposed mechanism for the IDH activity variation includes additional components regulating protein level. With the comparison of sequences from maize and teosinte (Zea mays ssp. Parviglumis), the maize wild ancestor, we found that some CCM genes had also been targeted for selection during maize domestication. Conclusions/Significance: Our results demonstrate the efficacy of association mapping for dissecting natural variation in primary metabolic pathways. The considerable genetic diversity observed in maize CCM genes underlies heritable phenotypic variation in enzyme activities and can be useful to identify putative functional sites.

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Language(s): eng - English
 Dates: 2010-04-012010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000276418200060
DOI: 10.1371/Journal.Pone.0009991
ISSN: 1932-6203 (Electronic) 1932-6203 (Linking)
URI: ://000276418200060 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2848677/pdf/pone.0009991.pdf?tool=pmcentrez
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 5 (4) Sequence Number: - Start / End Page: e9991 Identifier: -