English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Tricarboxylic Acid Cycle Activity Regulates Tomato Root Growth via Effects on Secondary Cell Wall Production

van der Merwe, M. J., Osorio, S., Araujo, W. L., Balbo, I., Nunes-Nesi, A., Maximova, E., et al. (2010). Tricarboxylic Acid Cycle Activity Regulates Tomato Root Growth via Effects on Secondary Cell Wall Production. Plant Physiology, 153(2), 611-621. doi:10.1104/pp.109.149047.

Item is

Files

show Files
hide Files
:
van der Merwe-2010-Tricarboxylic Acid C.pdf (Any fulltext), 771KB
Name:
van der Merwe-2010-Tricarboxylic Acid C.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
van der Merwe, M. J.1, Author              
Osorio, S.1, Author              
Araujo, W. L.1, Author              
Balbo, I.1, Author              
Nunes-Nesi, A.1, Author              
Maximova, E.2, Author              
Carrari, F.1, Author              
Bunik, V. I.3, Author
Persson, S.4, Author              
Fernie, A. R.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753304              
3External Organizations, ou_persistent22              
4Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

Content

show
hide
Free keywords: growing potato-tubers electron-transfer flavoprotein plant-growth plastidial phosphoglucomutase 2-oxoglutarate dehydrogenase photosynthetic performance nitrogen assimilation arabidopsis-thaliana malate-dehydrogenase cellulose synthesis
 Abstract: Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-C-14] glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.

Details

show
hide
Language(s): eng - English
 Dates: 2010-02-022010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000278340200024
DOI: 10.1104/pp.109.149047
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000278340200024http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879791/pdf/611.pdf?tool=pmcentrez
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 153 (2) Sequence Number: - Start / End Page: 611 - 621 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438