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  Tricarboxylic Acid Cycle Activity Regulates Tomato Root Growth via Effects on Secondary Cell Wall Production

van der Merwe, M. J., Osorio, S., Araujo, W. L., Balbo, I., Nunes-Nesi, A., Maximova, E., et al. (2010). Tricarboxylic Acid Cycle Activity Regulates Tomato Root Growth via Effects on Secondary Cell Wall Production. Plant Physiology, 153(2), 611-621. doi:10.1104/pp.109.149047.

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 Creators:
van der Merwe, M. J.1, Author           
Osorio, S.1, Author           
Araujo, W. L.1, Author           
Balbo, I.1, Author           
Nunes-Nesi, A.1, Author           
Maximova, E.2, Author           
Carrari, F.1, Author           
Bunik, V. I.3, Author
Persson, S.4, Author           
Fernie, A. R.1, Author           
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2Microscopy, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753304              
3External Organizations, ou_persistent22              
4Plant Cell Walls - Persson, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753318              

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Free keywords: growing potato-tubers electron-transfer flavoprotein plant-growth plastidial phosphoglucomutase 2-oxoglutarate dehydrogenase photosynthetic performance nitrogen assimilation arabidopsis-thaliana malate-dehydrogenase cellulose synthesis
 Abstract: Transgenic tomato (Solanum lycopersicum 'Moneymaker') plants independently expressing fragments of various genes encoding enzymes of the tricarboxylic acid cycle in antisense orientation have previously been characterized as exhibiting altered root growth. In this study, we evaluate the rates of respiration of roots from these lines in addition to determining their total dry weight accumulation. Given that these features were highly correlated, we decided to carry out an evaluation of the cell wall composition in the transformants that revealed a substantial reduction in cellulose. Since the bulk of cellulose is associated with the secondary cell walls in roots, we reasoned that the transformants most likely were deficient in secondary wall cellulose production. Consistent with these findings, cross-sections of the root collar (approximately 15 mm from the junction between root and stem) displayed reduced lignified secondary cell walls for the transformants. In contrast, cell and cell wall patterning displayed no differences in elongating cells close to the root tip. To further characterize the modified cell wall metabolism, we performed feeding experiments in which we incubated excised root tips in [U-C-14] glucose in the presence or absence of phosphonate inhibitors of the reaction catalyzed by 2-oxoglutarate dehydrogenase. Taken together, the combined results suggest that restriction of root respiration leads to a deficit in secondary cell wall synthesis. These data are discussed in the context of current models of biomass partitioning and plant growth.

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Language(s): eng - English
 Dates: 2010-02-022010
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000278340200024
DOI: 10.1104/pp.109.149047
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000278340200024http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2879791/pdf/611.pdf?tool=pmcentrez
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 153 (2) Sequence Number: - Start / End Page: 611 - 621 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438