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  Downregulation of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose-phosphate levels

van der Merwe, M. J., Groenewald, J. H., Stitt, M., Kossmann, J., & Botha, F. C. (2010). Downregulation of pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase activity in sugarcane culms enhances sucrose accumulation due to elevated hexose-phosphate levels. Planta, 231(3), 595-608. doi:10.1007/s11248-007-9079-x.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-22D8-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0017-BDB3-F
Genre: Journal Article

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 Creators:
van der Merwe, M. J.1, Author              
Groenewald, J. H.1, Author
Stitt, M.2, Author              
Kossmann, J.1, Author              
Botha, F. C.1, Author
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1External Organizations, ou_persistent22              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              

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Free keywords: metabolite profiling pfp substrate cycle sucrose sugarcane strongly decreased expression escherichia-coli pyrophosphatase transgenic potato plants fructose 2,6-bisphosphate fructose-6-phosphate 1-phosphotransferase subcellular compartmentation inorganic-phosphate solanum-tuberosum triose-phosphate tobacco plants
 Abstract: Analyses of transgenic sugarcane clones with 45-95% reduced cytosolic pyrophosphate: d-fructose-6-phosphate 1-phosphotransferase (PFP, EC 2.7.1.90) activity displayed no visual phenotypical change, but significant changes were evident in in vivo metabolite levels and fluxes during internode development. In three independent transgenic lines, sucrose concentrations increased between three- and sixfold in immature internodes, compared to the levels in the wildtype control. There was an eightfold increase in the hexose-phosphate:triose-phosphate ratio in immature internodes, a significant restriction in the triose phosphate to hexose phosphate cycle and significant increase in sucrose cycling as monitored by C-13 nuclear magnetic resonance. This suggests that an increase in the hexose-phosphate concentrations resulting from a restriction in the conversion of hexose phosphates to triose phosphates drive sucrose synthesis in the young internodes. These effects became less pronounced as the tissue matured. Decreased expression of PFP also resulted in an increase of the ATP/ADP and UTP/UDP ratios, and an increase of the total uridine nucleotide and, at a later stage, the total adenine nucleotide pool, revealing strong interactions between PPi metabolism and general energy metabolism. Finally, decreased PFP leads to a reduction of PPi levels in older internodes indicating that in these developmental stages PFP acts in the gluconeogenic direction. The lowered PPi levels might also contribute to the absence of increases in sucrose contents in the more mature tissues of transgenic sugarcane with reduced PFP activity.

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Language(s): eng - English
 Dates: 2007-04-142010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000273626500010
DOI: 10.1007/s11248-007-9079-x
ISSN: 0962-8819 (Print)0962-8819 (Linking)
URI: ://000273626500010http://www.springerlink.com/content/121152k636w71484/fulltext.pdf
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Title: Planta
Source Genre: Journal
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Pages: - Volume / Issue: 231 (3) Sequence Number: - Start / End Page: 595 - 608 Identifier: -