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  Mild reductions in cytosolic NADP-dependent isocitrate dehydrogenase activity result in lower amino acid contents and pigmentation without impacting growth

Sulpice, R., Sienkiewicz-Porzucek, A., Osorio, S., Krahnert, I., Stitt, M., Fernie, A. R., et al. (2010). Mild reductions in cytosolic NADP-dependent isocitrate dehydrogenase activity result in lower amino acid contents and pigmentation without impacting growth. Amino Acids, 39(4), 1055-1066. doi:10.1007/s00726-010-0617-0.

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Sulpice, R.1, Author              
Sienkiewicz-Porzucek, A.2, Author              
Osorio, S.2, Author              
Krahnert, I.2, Author              
Stitt, M.1, Author              
Fernie, A. R.2, Author              
Nunes-Nesi, A.2, Author              
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: Amino Acids/*analysis Cell Respiration Citric Acid Cycle/physiology Cytosol/enzymology/metabolism DNA, Complementary Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Isocitrate Dehydrogenase/*metabolism Ketoglutaric Acids/metabolism Lycopersicon esculentum/*genetics/growth & development/*metabolism NADP/metabolism Nitrogen/metabolism *Photosynthesis/genetics Pigmentation Pigments, Biological/metabolism Plant Leaves/enzymology/metabolism Plants, Genetically Modified/metabolism Polymerase Chain Reaction RNA, Plant/genetics
 Abstract: Transgenic tomato (Solanum lycopersicum) plants were generated targeting the cytosolic NADP-dependent isocitrate dehydrogenase gene (SlICDH1) via the RNA interference approach. The resultant transformants displayed a relatively mild reduction in the expression and activity of the target enzyme in the leaves. However, biochemical analyses revealed that the transgenic lines displayed a considerable shift in metabolism, being characterized by decreases in the levels of the TCA cycle intermediates, total amino acids, photosynthetic pigments, starch and NAD(P)H. The plants showed little change in photosynthesis with the exception of a minor decrease in maximum photosynthetic efficiency (F (v)/F (m)), and a small decrease in growth compared to the wild type. These results reveal that even small changes in cytosolic NADP-dependent isocitrate dehydrogenase activity lead to noticeable alterations in the activities of enzymes involved in primary nitrate assimilation and in the synthesis of 2-oxoglutarate derived amino acids. These data are discussed within the context of current models for the role of the various isoforms of isocitrate dehydrogenase within plant amino acid metabolism.

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Language(s): eng - English
 Dates: 2010-05-182010
 Publication Status: Published in print
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Title: Amino Acids
Source Genre: Journal
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Publ. Info: Wien : Springer
Pages: - Volume / Issue: 39 (4) Sequence Number: - Start / End Page: 1055 - 1066 Identifier: ISSN: 0939-4451
CoNE: https://pure.mpg.de/cone/journals/resource/954925571881