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  Developmental Stage Specificity and the Role of Mitochondrial Metabolism in the Response of Arabidopsis Leaves to Prolonged Mild Osmotic Stress

Skirycz, A., de Bodt, S., Obata, T., de Clercq, I., Claeys, H., de Rycke, R., et al. (2010). Developmental Stage Specificity and the Role of Mitochondrial Metabolism in the Response of Arabidopsis Leaves to Prolonged Mild Osmotic Stress. Plant Physiology, 152(1), 226-244. doi:10.1104/pp.109.148965.

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 Creators:
Skirycz, A.1, Author
de Bodt, S.1, Author
Obata, T.2, Author              
de Clercq, I.1, Author
Claeys, H.1, Author
de Rycke, R.1, Author
Andriankaja, M.1, Author
van Aken, O.1, Author
van Breusegem, F.1, Author
Fernie, A. R.2, Author              
Inze, D.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

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Free keywords: genome-wide analysis model data-analysis plant-cell-walls probe level data gene-expression water-deficit leaf growth oxidative stress drought stress transcription factor
 Abstract: When subjected to stress, plants reprogram their growth by largely unknown mechanisms. To provide insights into this process, the growth of Arabidopsis (Arabidopsis thaliana) leaves that develop under mild osmotic stress was studied. Early during leaf development, cell number and size were reduced by stress, but growth was remarkably adaptable, as division and expansion rates were identical to controls within a few days of leaf initiation. To investigate the molecular basis of the observed adaptability, leaves with only proliferating, exclusively expanding, and mature cells were analyzed by transcriptomics and targeted metabolomics. The stress response measured in growing and mature leaves was largely distinct; several hundred transcripts and multiple metabolites responded exclusively in the proliferating and/or expanding leaves. Only a few genes were differentially expressed across the three stages. Data analysis showed that proliferation and expansion were regulated by common regulatory circuits, involving ethylene and gibberellins but not abscisic acid. The role of ethylene was supported by the analysis of ethylene-insensitive mutants. Exclusively in proliferating cells, stress induced genes of the so-called "mitochondrial dysfunction regulon," comprising alternative oxidase. Up-regulation for eight of these genes was confirmed with promoter: beta-glucuronidase reporter lines. Furthermore, mitochondria of stress-treated dividing cells were morphologically distinct from control ones, and growth of plants overexpressing the alternative oxidase gene was more tolerant to osmotic and drought stresses. Taken together, our data underline the value of analyzing stress responses in development and demonstrate the importance of mitochondrial respiration for sustaining cell proliferation under osmotic stress conditions.

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Language(s): eng - English
 Dates: 2009-11-132010
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: ISI: ISI:000273148100018
DOI: 10.1104/pp.109.148965
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000273148100018http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799359/pdf/pp1520226.pdf?tool=pmcentrez
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Title: Plant Physiology
  Other : Plant Physiol.
Source Genre: Journal
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Publ. Info: Bethesda, Md. : American Society of Plant Biologists
Pages: - Volume / Issue: 152 (1) Sequence Number: - Start / End Page: 226 - 244 Identifier: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438