Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Developmental Stage Specificity and the Role of Mitochondrial Metabolism in the Response of Arabidopsis Leaves to Prolonged Mild Osmotic Stress

Skirycz, A., de Bodt, S., Obata, T., de Clercq, I., Claeys, H., de Rycke, R., et al. (2010). Developmental Stage Specificity and the Role of Mitochondrial Metabolism in the Response of Arabidopsis Leaves to Prolonged Mild Osmotic Stress. Plant Physiology, 152(1), 226-244. doi:10.1104/pp.109.148965.

Item is

Dateien

einblenden: Dateien
ausblenden: Dateien
:
Skirycz-2010-Developmental Stage.pdf (beliebiger Volltext), 2MB
Name:
Skirycz-2010-Developmental Stage.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Skirycz, A.1, Autor
de Bodt, S.1, Autor
Obata, T.2, Autor           
de Clercq, I.1, Autor
Claeys, H.1, Autor
de Rycke, R.1, Autor
Andriankaja, M.1, Autor
van Aken, O.1, Autor
van Breusegem, F.1, Autor
Fernie, A. R.2, Autor           
Inze, D.1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Inhalt

einblenden:
ausblenden:
Schlagwörter: genome-wide analysis model data-analysis plant-cell-walls probe level data gene-expression water-deficit leaf growth oxidative stress drought stress transcription factor
 Zusammenfassung: When subjected to stress, plants reprogram their growth by largely unknown mechanisms. To provide insights into this process, the growth of Arabidopsis (Arabidopsis thaliana) leaves that develop under mild osmotic stress was studied. Early during leaf development, cell number and size were reduced by stress, but growth was remarkably adaptable, as division and expansion rates were identical to controls within a few days of leaf initiation. To investigate the molecular basis of the observed adaptability, leaves with only proliferating, exclusively expanding, and mature cells were analyzed by transcriptomics and targeted metabolomics. The stress response measured in growing and mature leaves was largely distinct; several hundred transcripts and multiple metabolites responded exclusively in the proliferating and/or expanding leaves. Only a few genes were differentially expressed across the three stages. Data analysis showed that proliferation and expansion were regulated by common regulatory circuits, involving ethylene and gibberellins but not abscisic acid. The role of ethylene was supported by the analysis of ethylene-insensitive mutants. Exclusively in proliferating cells, stress induced genes of the so-called "mitochondrial dysfunction regulon," comprising alternative oxidase. Up-regulation for eight of these genes was confirmed with promoter: beta-glucuronidase reporter lines. Furthermore, mitochondria of stress-treated dividing cells were morphologically distinct from control ones, and growth of plants overexpressing the alternative oxidase gene was more tolerant to osmotic and drought stresses. Taken together, our data underline the value of analyzing stress responses in development and demonstrate the importance of mitochondrial respiration for sustaining cell proliferation under osmotic stress conditions.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2009-11-132010
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: ISI: ISI:000273148100018
DOI: 10.1104/pp.109.148965
ISSN: 1532-2548 (Electronic)0032-0889 (Linking)
URI: ://000273148100018http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2799359/pdf/pp1520226.pdf?tool=pmcentrez
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Plant Physiology
  Andere : Plant Physiol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Bethesda, Md. : American Society of Plant Biologists
Seiten: - Band / Heft: 152 (1) Artikelnummer: - Start- / Endseite: 226 - 244 Identifikator: ISSN: 0032-0889
CoNE: https://pure.mpg.de/cone/journals/resource/991042744294438