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  Mild Reductions in Mitochondrial NAD-Dependent Isocitrate Dehydrogenase Activity Result in Altered Nitrate Assimilation and Pigmentation But Do Not Impact Growth

Sienkiewicz-Porzucek, A., Sulpice, R., Osorio, S., Krahnert, I., Leisse, A., Urbanczyk-Wochniak, E., et al. (2010). Mild Reductions in Mitochondrial NAD-Dependent Isocitrate Dehydrogenase Activity Result in Altered Nitrate Assimilation and Pigmentation But Do Not Impact Growth. Molecular Plant, 3(1), 156-173. doi:10.1093/mp/ssp101.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2320-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0015-16AD-9
Genre: Journal Article

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 Creators:
Sienkiewicz-Porzucek, A.1, Author              
Sulpice, R.2, Author              
Osorio, S.1, Author              
Krahnert, I.1, Author              
Leisse, A.3, Author              
Urbanczyk-Wochniak, E.1, Author              
Hodges, M.4, Author
Fernie, A. R.1, Author              
Nunes-Nesi, A.1, Author              
Affiliations:
1Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              
2System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
3Small Molecules, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753340              
4External Organizations, ou_persistent22              

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Free keywords: carbon metabolism nitrogen metabolism photorespiration photosynthesis mitochondria tomato plant ammonium assimilation transgenic tomato plants organic-acid metabolism glutamate-dehydrogenase nitrogen-metabolism gene-expression photosynthetic performance malate-dehydrogenase citrate synthase phosphoenolpyruvate carboxylase
 Abstract: Transgenic tomato (Solanum lycopersicum) plants were generated expressing a fragment of the mitochondrial NAD-dependent isocitrate dehydrogenase gene (SlIDH1) in the antisense orientation. The transgenic plants displayed a mild reduction in the activity of the target enzyme in the leaves but essentially no visible alteration in growth from the wild-type. Fruit size and yield were, however, reduced. These plants were characterized by relatively few changes in photosynthetic parameters, but they displayed a minor decrease in maximum photosynthetic efficiency (Fv/Fm). Furthermore, a clear reduction in flux through the tricarboxylic acid (TCA) cycle was observed in the transformants. Additionally, biochemical analyses revealed that the transgenic lines exhibited considerably altered metabolism, being characterized by slight decreases in the levels of amino acids, intermediates of the TCA cycle, photosynthetic pigments, starch, and NAD(P)H levels, but increased levels of nitrate and protein. Results from these studies show that even small changes in mitochondrial NAD-dependent isocitrate dehydrogenase activity lead to noticeable alterations in nitrate assimilation and suggest the presence of different strategies by which metabolism is reprogrammed to compensate for this deficiency.

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Language(s): eng - English
 Dates: 2009-12-262010
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: ISI:000273893200012
DOI: 10.1093/mp/ssp101
ISSN: 1674-2052 (Print)1674-2052 (Linking)
URI: ://000273893200012http://mplant.oxfordjournals.org/content/3/1/156.full.pdf
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Title: Molecular Plant
Source Genre: Journal
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Publ. Info: Oxford University Press
Pages: - Volume / Issue: 3 (1) Sequence Number: - Start / End Page: 156 - 173 Identifier: Other: 1674-2052
CoNE: https://pure.mpg.de/cone/journals/resource/1674-2052