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  Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana

Schliep, M., Ebert, B., Simon-Rosin, U., Zoeller, D., & Fisahn, J. (2010). Quantitative expression analysis of selected transcription factors in pavement, basal and trichome cells of mature leaves from Arabidopsis thaliana. Protoplasma, 241(1-4), 29-36. doi:10.1007/s00709-009-0099-7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2342-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0014-2343-1
Genre: Journal Article

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 Creators:
Schliep, M.1, Author
Ebert, B.2, Author              
Simon-Rosin, U.2, 3, Author              
Zoeller, D.2, Author              
Fisahn, J.2, Author              
Affiliations:
1External Organizations, ou_persistent22              
2Biophysical Analysis, Infrastructure Groups and Service Units, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753307              
3Molecular Plant Nutrition, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753321              

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Free keywords: arabidopsis thaliana real-time rt-pcr single cell transcription factors epidermis yabby gene family ectopic expression individual cells barley leaves differentiation caprice morphogenesis initiation mesophyll protein
 Abstract: Gene expression levels of several transcription factors from Arabidopsis thaliana that were described previously to be involved in leaf development and trichome formation were analysed in trichome, basal and pavement cells of mature leaves. Single cell samples of these three cells types were collected by glass micro-capillaries. Real-time reverse transcription (RT)-PCR was used to analyse expression patterns of the following transcription factors: MYB23, MYB55, AtHB1, FILAMENTOUS FLOWER (FIL)/YABBY1 (YAB1), TRIPTYCHON (TRY) and CAPRICE (CPC). A difference in the expression patterns of TRY and CPC was revealed. Contrary to the CPC expression pattern, no transcripts of TRY could be detected in pavement cells. FIL/YAB1 was exclusively expressed in trichome cells. AtHB1 was highly expressed throughout all three cell types. MYB55 was higher expressed in basal cells than in trichome and pavement cells. MYB23 showed a pattern of low expression in pavement cells, medium in basal cells and high expression in trichomes. Expression patterns obtained by single cell sampling and real-time RT-PCR were compared to promoter GUS fusions of the selected transcription factors. Therefore, we regenerated two transgenic Arabidopsis lines that expressed the GUS reporter gene under control of the promoters of MYB55 and YAB1. In conclusion, despite their function in leaf morphogenesis, all six transcription factors were detected in mature leaves. Furthermore, single cell sampling and promoter GUS staining patterns demonstrated the predominant presence of MYB55 in basal cells as compared to pavement cells and trichomes.

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Language(s): eng - English
 Dates: 2010-01-272010
 Publication Status: Published in print
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 Identifiers: ISI: ISI:000276899000004
DOI: 10.1007/s00709-009-0099-7
ISSN: 1615-6102 (Electronic) 0033-183X (Linking)
URI: ://000276899000004 http://www.springerlink.com/content/x7438g527nkhk776/fulltext.pdf
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Title: Protoplasma
Source Genre: Journal
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Pages: - Volume / Issue: 241 (1-4) Sequence Number: - Start / End Page: 29 - 36 Identifier: -